人抗菌肽LL-37在人鼻黏膜中的表达。
The expression of human antimicrobial peptide LL-37 in the human nasal mucosa.
作者信息
Chen Po-Hsu, Fang Sheen-Yie
机构信息
Department of Otolaryngology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
出版信息
Am J Rhinol. 2004 Nov-Dec;18(6):381-5.
BACKGROUND
LL-37, an antimicrobial peptide, has been discovered to be produced by a number of epithelial cells. It is identified as a key element in the innate host defense mechanism. Because little is known about the expression of LL-37 in human sinonasal tract, we conducted this study to investigate the expression of LL-37 in human nasal mucosa.
METHODS
We investigated the expression of LL-37 gene by the reverse transcription polymerase chain reaction (RT-PCR) and localization of LL-37 peptide by immunohistochemistry in the inferior turbinate mucosa of 7 normal subjects and the nasal polyps of 12 patients with chronic paranasal sinusitis.
RESULTS
The transcripts of the LL-37 gene were detected in all human nasal tissues analyzed by RT-PCR. There is a significant increase of LL-37 mRNA expression in nasal polyps as compared with the normal nasal mucosa. Using immunohistochemistry, LL-37 peptide was localized in surface epithelial cells and submucosal glands.
CONCLUSIONS
Our findings suggest that LL-37 is expressed by nasal mucosa and is upregulated during inflammation.
背景
抗菌肽LL-37已被发现由多种上皮细胞产生。它被认为是先天性宿主防御机制中的关键要素。由于对LL-37在人类鼻窦中的表达了解甚少，我们开展了这项研究以调查LL-37在人鼻黏膜中的表达。
方法
我们采用逆转录聚合酶链反应（RT-PCR）检测7名正常受试者下鼻甲黏膜及12例慢性鼻窦炎患者鼻息肉中LL-37基因的表达，并通过免疫组织化学法对LL-37肽进行定位。
结果
通过RT-PCR在所有分析的人鼻组织中均检测到LL-37基因的转录本。与正常鼻黏膜相比，鼻息肉中LL-37 mRNA表达显著增加。采用免疫组织化学法，LL-37肽定位于表面上皮细胞和黏膜下腺。
结论
我们的研究结果表明，LL-37由鼻黏膜表达，并在炎症过程中上调。
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