坦桑尼亚法医尸检案例中血液和组织中抗疟药氯喹的高效液相色谱分析。

HPLC analysis of anti-malaria agent, chloroquine in blood and tissue from forensic autopsy cases in Tanzania.

作者信息

Yonemitsu Kosei, Koreeda Ako, Kibayashi Kazuhiko, Ng'walali Paul, Mbonde Martin, Kitinya James, Tsunenari Shigeyuki

机构信息

Department of Forensic Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.

出版信息

Leg Med (Tokyo). 2005 Mar;7(2):113-6. doi: 10.1016/j.legalmed.2004.10.002.

DOI:10.1016/j.legalmed.2004.10.002

PMID:15708335

Abstract

HPLC analysis of anti-malaria agent, chloroquine (CQ) in blood and tissues with a simple HCl back extraction method was applied to three forensic autopsy cases in Dar es Salaam, Tanzania. CQ concentrations in femoral vein blood were 8.5, 48.4 and 43.8 microg/ml in three cases, respectively, which were high enough to attribute the cause of deaths to an acute CQ poisoning. There were great site dependent variations in blood CQ levels. The right heart blood samples were very high, which may be explained by incomplete distribution of the drug before death or postmortem diffusion from liver and its surrounding blood, as high CQ levels were remarkable in the liver. Suicidal and accidental CQ poisonings are very common and CQ is a very important chemical in the field of forensic toxicology in Tanzania.

摘要

采用简单的盐酸反萃取法，通过高效液相色谱法对坦桑尼亚达累斯萨拉姆三例法医尸检案例中的血液和组织中的抗疟药氯喹（CQ）进行分析。三例案例中股静脉血中CQ浓度分别为8.5、48.4和43.8微克/毫升，高到足以将死亡原因归为急性CQ中毒。血液中CQ水平存在很大的部位依赖性差异。右心血液样本中的含量非常高，这可能是由于死亡前药物分布不完全或死后从肝脏及其周围血液扩散所致，因为肝脏中CQ水平很高。自杀性和意外性CQ中毒非常常见，CQ是坦桑尼亚法医毒理学领域一种非常重要的化学物质。