Machackova-Kopecna M, Bartos M, Straka M, Ludvik V, Svastova P, Alvarez J, Lamka J, Trcka I, Treml F, Parmova I, Pavlik I
Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.
Vet Microbiol. 2005 Feb 25;105(3-4):261-8. doi: 10.1016/j.vetmic.2004.11.008. Epub 2005 Jan 22.
As the attempt to eradicate paratuberculosis in one red deer (Cervus elaphus) farm failed, all 167 red deer of different age groups were slaughtered and examined by culture for mycobacteria, and the farm was closed down. Spleen and hepatic lymph nodes, mediastinal lymph node, ileocecal lymph node, and ileum were collected from each animal and examined (a total of 835 organs). Neither tuberculosis lesions nor pathognomic signs of paratuberculosis were detected. Among all microscopically negative for mycobacteria organs, Mycobacterium avium subsp. paratuberculosis alone was isolated from 165 organs, M. a. avium alone from 41 organs, and both pathogens from four organs. M. a. paratuberculosis alone was detected in 71 red deer, M. a. avium alone in 13 red deer and both pathogens in 18 red deer. Using standardised RFLP methods, three IS900 RFLP types B-C1, B-C16, and B-C32 were identified among 40 M. a. paratuberculosis isolates and four IS901 RFLP types N-B1, N-B3, N-B4, and P-B3 among 17 M. a. avium isolates.
由于在一个马鹿（ Cervus elaphus ）养殖场根除副结核病的尝试失败，所有167只不同年龄组的马鹿被屠宰，并通过培养检测分枝杆菌，该养殖场被关闭。从每只动物身上采集脾脏、肝淋巴结、纵隔淋巴结、回盲部淋巴结和回肠并进行检查（共835个器官）。未检测到结核病变或副结核病的特征性体征。在所有分枝杆菌镜检阴性的器官中，仅从165个器官中分离出副结核分枝杆菌亚种，仅从41个器官中分离出鸟分枝杆菌，从4个器官中分离出两种病原体。仅在71只马鹿中检测到副结核分枝杆菌亚种，仅在13只马鹿中检测到鸟分枝杆菌，在18只马鹿中检测到两种病原体。使用标准化的RFLP方法，在40株副结核分枝杆菌分离株中鉴定出三种IS900 RFLP类型B-C1、B-C16和B-C32，在17株鸟分枝杆菌分离株中鉴定出四种IS901 RFLP类型N-B1、N-B3、N-B4和P-B3。
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