口腔扁平苔藓患者不同口腔液中促炎的、NF-κB依赖性细胞因子：肿瘤坏死因子-α、白细胞介素-1-α、白细胞介素-6和白细胞介素-8的比较。

A comparison of the pro-inflammatory, NF-kappaB-dependent cytokines: TNF-alpha, IL-1-alpha, IL-6, and IL-8 in different oral fluids from oral lichen planus patients.

作者信息

Rhodus Nelson L, Cheng Bin, Myers Sandra, Bowles Walter, Ho Vu, Ondrey Frank

机构信息

Division of Oral Medicine, Oral Diagnosis and Radiology, Department of Diagnostic and Surgical Sciences, School of Dentistry, University of Minnesota, 7-536 Moos Tower, 515 Delaware Street S.E., Minneapolis, MN 55455, USA.

出版信息

Clin Immunol. 2005 Mar;114(3):278-83. doi: 10.1016/j.clim.2004.12.003.

DOI:10.1016/j.clim.2004.12.003

PMID:15721838

Abstract

To explore the feasibility of detection of the level of NF-kappaB-dependent cytokines in oral fluids from patient with oral lichen planus (OLP) for clinical application, 13 OLP subjects were enrolled in the study as were 13 age-sex-matched controls. In each subject, the whole unstimulated saliva (WUS), mixture of saliva and isotonic saline oral rinse (Saliva-NaCl), and lesion tissue transudates (TT) were collected by standard techniques. The level of cytokines, TNF-alpha, IL-1-alpha, IL-6, and IL-8 in three types of oral fluids was determined by ELISA. In the three types of oral fluids, a significantly higher level of these cytokines was detected in OLP patients than in normal controls. These results indicate that NF-kappaB-dependent inflammatory cytokines may be detected at increased levels in certain oral fluids which may have diagnostic and prognostic potential for monitoring disease activity and making therapeutic decisions in patients with OLP.

摘要

为探讨检测口腔扁平苔藓（OLP）患者口腔液中NF-κB依赖性细胞因子水平用于临床应用的可行性，本研究招募了13名OLP受试者以及13名年龄和性别匹配的对照者。采用标准技术收集每位受试者的全唾液（WUS）、唾液与等渗盐水口腔冲洗液的混合物（唾液-氯化钠）以及病损组织渗出液（TT）。通过酶联免疫吸附测定法（ELISA）测定三种口腔液中细胞因子肿瘤坏死因子-α（TNF-α）、白细胞介素-1-α（IL-1-α）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的水平。在这三种口腔液中，OLP患者体内这些细胞因子的水平显著高于正常对照者。这些结果表明，在某些口腔液中可检测到NF-κB依赖性炎性细胞因子水平升高，这可能对监测OLP患者的疾病活动及做出治疗决策具有诊断和预后潜力。