Karasov Ariela O, Boothroyd John C, Arrizabalaga Gustavo
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Int J Parasitol. 2005 Mar;35(3):285-91. doi: 10.1016/j.ijpara.2004.11.015. Epub 2005 Jan 21.
Na+/H+ exchangers (NHEs) are ubiquitous membrane proteins that catalyze the exchange of Na+ for H+ and are critical in pH and cell volume regulation, as well as osmotolerance. In this study, we identify and characterize a novel NHE, TgNHE2, in Toxoplasma gondii. Immunofluorescence studies show that TgNHE2 is localized to the rhoptries, secretory organelles involved in invasion. TgNHE2 is the first intracellular NHE to be characterized in a protozoan parasite and its localization suggests possible roles for the rhoptries in osmotolerance and/or as secretory lysosomes-like granules.
钠/氢交换体（NHEs）是普遍存在的膜蛋白，可催化钠与氢的交换，对pH值、细胞体积调节以及渗透压耐受性至关重要。在本研究中，我们鉴定并表征了刚地弓形虫中的一种新型NHE，即TgNHE2。免疫荧光研究表明，TgNHE2定位于棒状体，这是参与入侵的分泌细胞器。TgNHE2是首个在原生动物寄生虫中得到表征的细胞内NHE，其定位表明棒状体在渗透压耐受性和/或作为分泌性溶酶体样颗粒方面可能发挥的作用。
Zotero 插件