在一种小沟结合铂类抗肿瘤药物与Hoechst染料33258之间观察到独特的协同结合相互作用。
Unique cooperative binding interaction observed between a minor groove binding Pt antitumor agent and Hoechst dye 33258.
作者信息
Harris Amanda, Qu Yun, Farrell Nicholas
机构信息
Department of Chemistry, Virginia Commonwealth University, 1001 W. Main Street, Richmond, Virginia 23284-2006, USA.
出版信息
Inorg Chem. 2005 Mar 7;44(5):1196-8. doi: 10.1021/ic048356p.
The trinuclear compound [{Pt(NH3)3}2mu-{trans-Pt(NH3)2(H2N(CH2)6NH2)}2(6+) (0,0,0/t,t,t) binds to DNA only through noncovalent hydrogen bonding and electrostatic interactions. The presence of this 6+ cation allows discrimination of binding modes for common DNA ligands: binding of minor-groove agents such as Hoechst 33258 is cooperative, and dye-DNA interaction is enhanced whereas intercalation as exemplified by ethidium bromide is competitively inhibited.
三核化合物[{Pt(NH₃)₃}₂μ-{反式-Pt(NH₃)₂(H₂N(CH₂)₆NH₂)}₂(6⁺) (0,0,0/t,t,t)]仅通过非共价氢键和静电相互作用与DNA结合。这种6⁺阳离子的存在使得能够区分常见DNA配体的结合模式：小沟结合剂如Hoechst 33258的结合是协同的，染料与DNA的相互作用增强，而以溴化乙锭为例的嵌入则受到竞争性抑制。
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