Liu Chen-Guang, Desai Kashappa Goud H, Chen Xi-Guang, Park Hyun-Jin
Life Science College, Ocean University of China, Qingdao 266003, People's Republic of China.
J Agric Food Chem. 2005 Mar 9;53(5):1728-33. doi: 10.1021/jf040304v.
Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as cross-linker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticles containing trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GA concentration affected both the enzyme activity of the nanoparticle and particle size. Results indicated that the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increased with increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount of GA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentration of GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution were also studied. The results showed that the kinetic constant value (K(m)) of TR immobilized on nanoparticle (71.9 mg/mL) was higher than that of pure TR (50.2 mg/mL). However, the thermal stability and optimum temperature of TR immobilized on nanoparticles improved, which make it more attractive in the application aspect.
使用戊二醛（GA）作为交联剂，将胰蛋白酶固定在亚麻酸修饰的壳聚糖上，这通过傅里叶变换红外（FTIR）光谱得到证实。固定化胰蛋白酶经超声处理后可制备含胰蛋白酶（TR）的壳聚糖纳米颗粒。GA浓度影响纳米颗粒的酶活性和粒径。结果表明，固定在亚麻酸修饰的壳聚糖纳米颗粒上的胰蛋白酶活性随着GA浓度增加至0.07%（v/v）而升高，然后随着GA量的增加而降低。另一方面，随着GA浓度从0.03%增加到0.1%（v/v），粒径增大（从523 nm增大到1372 nm）。还研究了纳米颗粒溶液的酶催化特性。结果表明，固定在纳米颗粒上的TR的动力学常数（K(m)）值（71.9 mg/mL）高于纯TR（50.2 mg/mL）。然而，固定在纳米颗粒上的TR的热稳定性和最适温度提高，这使其在应用方面更具吸引力。
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