简短报告：墨西哥先天性弓形虫感染新生儿筛查试点研究

Short report: neonatal screening pilot study of Toxoplasma gondii congenital infection in Mexico.

作者信息

Vela-Amieva Marcela, Cañedo-Solares Irma, Gutiérrez-Castrellón Pedro, Pérez-Andrade Martha, González-Contreras Claudia, Ortíz-Cortés Joel, Ortega-Velázquez Venancio, Galván-Ramírez Maria de la Luz, Ruiz-García Matilde, Saltigeral-Simentel Patrica, Ordaz-Favila Juan Carlos, Sánchez Carmen, Correa Dolores

机构信息

Servicio de Genética de la Nutrición, Instituto Nacional de Pediatría, Secretaría de Salud, Mexico City, Mexico.

出版信息

Am J Trop Med Hyg. 2005 Feb;72(2):142-4.

PMID:15741549

Abstract

Congenital toxoplasmosis is an obstetric problem in Mexico, but its actual frequency is unknown. Using a network for screening of non-infectious disorders, we performed a pilot study to determine the frequency of IgM antibodies to Toxoplasma gondii in 1,003 infants (53.1% male, mean +/- SD age = 18.3 +/- 13.0 days, birth weight = 3.116 +/- 0.453 kg) in Mexico City from March to April 2003. Blood samples embedded in filter paper were assayed for IgM antibodies using a capture enzyme-linked immunosorbent assay and results were confirmed by Western blot. Two asymptomatic newborns, one of them premature, had IgM and IgG antibodies in a serum sample taken from both the infant and the mother and were clinically followed. Our data suggest a frequency of approximately two cases of congenital T. gondii infection per 1,000 newborns in Mexico City.

摘要

先天性弓形虫病在墨西哥是一个产科问题，但其实际发病率尚不清楚。利用一个非传染性疾病筛查网络，我们开展了一项试点研究，以确定2003年3月至4月间墨西哥城1003名婴儿（53.1%为男性，平均年龄±标准差=18.3±13.0天，出生体重=3.116±0.453千克）中抗弓形虫IgM抗体的发病率。使用捕获酶联免疫吸附测定法检测滤纸血样中的IgM抗体，并通过蛋白质印迹法确认结果。两名无症状新生儿，其中一名早产，在采集的婴儿及其母亲血清样本中均检测到IgM和IgG抗体，并对其进行临床随访。我们的数据表明，墨西哥城每1000名新生儿中先天性弓形虫感染病例约为两例。