孤雌生殖寄生蜂黄足侧沟茧蜂卵巢中酚氧化酶样活性及病毒样颗粒的功能

Phenoloxidase-like activities and the function of virus-like particles in ovaries of the parthenogenetic parasitoid Venturia canescens.

作者信息

Schmidt Otto, Li Dongmei, Beck Markus, Kinuthia Wanja, Bellati Judy, Roberts Harry L S

机构信息

Department of Applied and Molecular Ecology, Waite Campus, Adelaide University, Glen Osmond, SA 5064, Australia.

出版信息

J Insect Physiol. 2005 Feb;51(2):117-25. doi: 10.1016/j.jinsphys.2004.05.006.

DOI:10.1016/j.jinsphys.2004.05.006

PMID:15749097

Abstract

The ichneumonid endoparasitoid Venturia canescens successfully develops inside the hemocoel of another insect by using maternal protein secretions, including nucleic acid-free virus-like particles (VLPs), to manipulate host physiology. These VLPs consist of four major proteins, which are produced mainly in the calyx tissue and transferred into the host insect together with the egg. One of the protein-coding genes (vlp1), with similarities to phospholipid-hydroperoxide glutathione peroxidases (PHGPx), exists in allelic forms producing two protein variants with different protein properties. Here, we summarise observations indicating that oocytes and eggs are the source of reactive electrons, which potentially damage the lining and membranes of calyx tissues. We discuss the possible role of VLP1 in counteracting the damaging effects of oxidised phospholipids on membranes surrounding VLPs in the calyx lumen.

摘要

姬蜂内寄生蜂黄足侧沟茧蜂通过利用母体蛋白质分泌物，包括无核酸病毒样颗粒（VLPs），来操纵宿主生理机能，从而在另一种昆虫的血腔中成功发育。这些病毒样颗粒由四种主要蛋白质组成，主要在萼片组织中产生，并与卵一起转移到宿主昆虫体内。其中一个与磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）相似的蛋白质编码基因（vlp1），以等位基因形式存在，产生两种具有不同蛋白质特性的蛋白质变体。在这里，我们总结了一些观察结果，表明卵母细胞和卵是活性电子的来源，这些活性电子可能会损害萼片组织的内衬和膜。我们讨论了VLP1在抵消氧化磷脂对萼腔中病毒样颗粒周围膜的破坏作用方面可能发挥的作用。