Wang C-J, Chen C-C
Department of Botany, National Taiwan University, Taipei, Taiwan, Republic of China.
Cytogenet Genome Res. 2005;109(1-3):63-9. doi: 10.1159/000082383.
Cytogenetic maps depict the location and order of markers along chromosomes. Cytogenetic maps are important in genome research as they relate the genetic data and molecular sequences to the morphological features of chromosomes. In this paper, we discuss various methods used in cytogenetic mapping in maize, with special reference to fluorescence in situ hybridization (FISH) of single-copy sequences on meiotic pachytene chromosomes.
细胞遗传学图谱描绘了标记沿着染色体的位置和顺序。细胞遗传学图谱在基因组研究中很重要,因为它们将遗传数据和分子序列与染色体的形态特征联系起来。在本文中,我们讨论了玉米细胞遗传学图谱构建中使用的各种方法,特别提及了减数分裂粗线期染色体上单拷贝序列的荧光原位杂交(FISH)。