百合植物中存在争议的端粒。

The controversial telomeres of lily plants.

作者信息

de la Herrán R, Cuñado N, Navajas-Pérez R, Santos J L, Ruiz Rejón C, Garrido-Ramos M A, Ruiz Rejón M

机构信息

Departamento de Genética, Facultad de Ciencias, Universidad de Granada, Granada, Spain.

出版信息

Cytogenet Genome Res. 2005;109(1-3):144-7. doi: 10.1159/000082393.

DOI:10.1159/000082393

PMID:15753570

Abstract

The molecular structure of the exceptional telomeres of six plant species belonging to the order Asparagales and two species of the order Liliales was analyzed using Southern blot and fluorescence in situ hybridization. Three different situations were found, namely: i) In the two Liliales species, Tulipa australis (Liliaceae) and Merendera montana (Colchicaceae), the chromosome ends display hybridization signals with oligonucleotides resembling telomere repeats of both plants (TTTAGGG)n and vertebrates (TTAGGG)n. ii) Asparagales species such as Phormium tenax (Hemerocallidaceae), Muscari comosum (Hyacinthaceae), Narcissus jonquilla (Amaryllidaceae) and Allium sativum (Alliaceae) lack both the plant telomere repeats and the vertebrate telomere repeats. iii) Two other Asparagales species, Aloe vera (Asphodelaceae) and an Iris hybrid (Iridaceae), display positive hybridization with the vertebrate telomere repeats but not with the plant telomere repeats. Southern blot hybridization revealed concurring results. On this basis, the composition of the telomere structure in this plant group is discussed.

摘要

使用Southern印迹法和荧光原位杂交技术分析了天门冬目六个植物物种和百合目两个植物物种的特殊端粒的分子结构。发现了三种不同的情况，即：i）在百合目两个物种，即澳洲郁金香（百合科）和蒙大拿秋水仙（秋水仙科）中，染色体末端与类似于植物（TTTAGGG）n和脊椎动物（TTAGGG）n端粒重复序列的寡核苷酸显示杂交信号。ii）天门冬目物种，如新西兰麻（萱草科）、葡萄风信子（风信子科）、黄水仙（石蒜科）和大蒜（葱科），既缺乏植物端粒重复序列，也缺乏脊椎动物端粒重复序列。iii）另外两个天门冬目物种，库拉索芦荟（阿福花科）和一种鸢尾杂交种（鸢尾科），与脊椎动物端粒重复序列显示阳性杂交，但与植物端粒重复序列不显示阳性杂交。Southern印迹杂交揭示了一致的结果。在此基础上，讨论了该植物类群中端粒结构的组成。