Li Yanlin, Yang Hao, Han Rui, Li Linzhi, Li Xiuqun, Li Shihe, Zhao Xiaoxia
Department of Orthopedic Surgery, the First Affiliated Hospital of Kunming Medical Colledge, Kunming Yunnan, 650032, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2005 Feb;19(2):118-23.
To evaluate the osteogenesis of three bio-bone derived materials in repairing segmental bone defects.
Sixty Japanese rabbits were made 10 mm radius segmental defects and divided into 5 groups (groups A, B, C, D and E, n=12). Composite fully deproteinised bone (CFDB, group A), partially deproteinised bone (PDPB, group B), partially decalcified bone (PDCB, group C), autogenous iliac bone graft (group D) and no implant (group E) were implanted into the radius segmental bone defects of rabbits. The specimens were examined after 4, 8, 12 and 24 weeks; the osteogenesis was evaluated through X-ray radiograph and undecalcified solid tissue histological examination.
The border between the material and host's bone was distinct after 4 weeks and blurred after 8 weeks; the density of partial edge of the material was similar to that of radii after 12 weeks. The medullary cavity of bone reopened in group B; the density of most defect area was similar to that of the host bone and there was a few high density shadow in group C; the density of most defect area was higher than that of host bone in group A after 24 weeks. There was no significant difference in radiograph scoring between groups A, B and C after 4 weeks and 8 weeks (P>0.05); the scores of group B and C were higher than that of group A after 12 weeks (P<0.05); and the scores were arranged as follow: group D > group B > group C > group A after 24 weeks (P<0.05). Bone callus grew toward defect area and new bone adhered to the material after 4 weeks and 8 weeks; more new bone formed, and the materials were absorbed and degraded with time. The quantity of bone formation was more in group D than in group B and in group B than in group C and in group C than in group A after 24 weeks (P<0.05).
PDPB had good osteogenesis in repairing the segmental bone defect, PDCB was inferior to it, both PDPB and PDCB are fit to repair segmental bone defect. Both of them were inferior to autogenous bone.
评估三种生物骨衍生材料修复节段性骨缺损的成骨能力。
将60只日本大耳白兔制成半径为10mm的节段性骨缺损，并分为5组（A组、B组、C组、D组和E组，每组12只）。将复合完全脱蛋白骨（CFDB，A组）、部分脱蛋白骨（PDPB，B组）、部分脱钙骨（PDCB，C组）、自体髂骨移植（D组）和不植入任何材料（E组）植入兔桡骨节段性骨缺损处。分别在4周、8周、12周和24周后对标本进行检查；通过X线片和不脱钙实体组织组织学检查评估成骨情况。
4周后材料与宿主骨之间的边界清晰，8周后边界模糊；12周后材料部分边缘的密度与桡骨密度相似。B组骨的髓腔重新开放；C组大部分缺损区域的密度与宿主骨相似，有少量高密度影；24周后A组大部分缺损区域的密度高于宿主骨。4周和8周时，A组、B组和C组的X线评分差异无统计学意义（P>0.05）；12周时，B组和C组的评分高于A组（P<0.05）；24周时评分排序为：D组>B组>C组>A组（P<0.05）。4周和8周时骨痂向缺损区域生长，新骨附着于材料；随着时间的推移，形成更多新骨，材料被吸收降解。24周时，D组的骨形成量多于B组，B组多于C组，C组多于A组（P<0.05）。
PDPB在修复节段性骨缺损方面具有良好的成骨能力，PDCB次之，二者均适合修复节段性骨缺损，但均不如自体骨。
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