[肝再生增强因子在肝癌细胞中的表达及其临床意义]
[Expression of augmenter of liver regeneration in hepatic tumor cells and its clinical significance].
作者信息
Sun Hang, Yu Hui-feng, Wu Chuan-xin, Guan Xiao-qin, Liu Qi
机构信息
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2005 Mar;13(3):205-8.
OBJECTIVE
To investigate the effects of augmenter of liver regeneration (ALR) on the proliferation of hepatocytes and hepatic tumor cells and the expression of ALR in herpatocellular carcinoma (HCC).
METHODS
Primary rat hepatocytes, QGY and HepG2 cells were cultured separately with ALR from different species. Cell proliferation was detected by their 3H-TdR uptake. The expression of ALR was examined in 9 normal hepatic tissues and 21 HCC cases using immunohistochemistry method.
RESULTS
Different ALRs could stimulate the proliferation of HepG2 and QGY cells in a dose-dependent way in vitro, but all ALR had no influence in the proliferation of primary rat hepatocytes. The expression of ALR was absent in normal hepatic tissues, but present in all HCC hepatic tissues. However, the expression of ALR had no relationship with the differentiation and size of the carcinomas.
CONCLUSION
ALR might play an important role in the occurrence and development of HCC.
目的
研究肝再生增强因子（ALR）对肝细胞和肝肿瘤细胞增殖的影响以及其在肝细胞癌（HCC）中的表达情况。
方法
将原代大鼠肝细胞、QGY细胞和HepG2细胞分别与不同种属的ALR共同培养。通过检测其3H-TdR摄取来检测细胞增殖。采用免疫组织化学方法检测9例正常肝组织和21例HCC病例中ALR的表达。
结果
不同的ALR在体外可呈剂量依赖性地刺激HepG2和QGY细胞增殖，但所有ALR对原代大鼠肝细胞的增殖均无影响。正常肝组织中无ALR表达，但所有HCC肝组织中均有表达。然而，ALR的表达与癌的分化程度和大小无关。
结论
ALR可能在HCC的发生发展中起重要作用。
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