[Optical properties of human normal bladder tissue at five different wavelengths of laser and their linearly polarized laser irradiation in vitro].

A double-integrating-spheres system, the basic principle of measuring technology of radiation, and an optical model of biological tissues were used for the study. Optical properties of human normal bladder tissue at 476.5, 488, 496.5, 514.5 and 532 nm of laser and their linearly polarized laser irradiation were studied. The results of measurement showed that total attenuation coefficient and scattering coefficient of human normal bladder tissue at these wavelengths of laser and their linearly polarized laser irradiation increased with decreasing wavelengths. And these was an obvious distinction between the results at these wavelengths of laser and their linearly polarized laser irradiation. Absorption coefficient of human normal bladder tissue at these wavelengths of laser and their linearly polarized laser irradiation was tardily increased with decreasing wavelengths. But there were a number of gurgitations. And these were independent of the wavelengths of laser or their linearly polarized laser irradiation. Mean cosine of scattering of human normal bladder tissue at these wavelengths of laser and their linearly polarized laser irradiation also increased with decreasing wavelengths. And these was an obvious distinction with these wavelengths of laser and their linearly polarized laser irradiation. But penetration depth of human normal bladder tissue at these wavelengths of laser and their linearly polarized laser irradiation also increased with increasing wavelengths. But there were a number of gurgitations. Refractive index of human normal bladder tissue at these wavelengths of laser ranged from 1.37 to 1.44. Absorption coefficient, scattering coefficient, total attenuation coefficient, and effective attenuation coefficients of human normal bladder tissue in Kubelka-Munk two-flux model at the same wavelength of laser and the linearly polarized laser irradiation do not exhibit prominent distinction (P > 0.05). Some absorption coefficient, scattering coefficient, total attenuation coefficient, and effective attenuation coefficient of human normal bladder tissue in Kubelka-Munk two-flux model at different wavelengths of laser and their linearly polarized laser irradiation exhibit obvious distinction.