基于2,4-二氯苯基荧光酮荧光猝灭法测定中草药和茶叶中的铁(III)

[Determination of iron(III) in Chinese herbal medicine and tea based on fluorescence quenching of 2,4-dichro-phenylfluorone].

作者信息

Dai Gang, Aodeng Gao-wa

机构信息

Institute of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010021, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jul;24(7):848-50.

PMID:15766088

Abstract

A new spectrofluorimetric method for the determination of trace amount of Fe(III) has been developed. This method is based on the fluorescence quenching of 2,4-dichro-phenylfluorone due to the formation of complex Fe(III)-DCIPF. In pH 5.2-5.9 buffer solution, Fe (III) and DCIPF react on each other to form a red complex. Its composition was established by method of continuous variations and molar-ratio as Fe(III): DCIPF = 1:4, the excitation and emission wavelengths were found to be 282 and 560 nm, respectively. There is a linear relationship in the range 4-24 ng x mL(-1) for Fe(III). This method is simple and rapid, and has been applied to the determination of trace iron in Chinese herbal medicine and tea samples with satisfactory results.

摘要

已开发出一种用于测定痕量铁（III）的新的荧光分光光度法。该方法基于由于形成铁（III）-2,4-二氯苯基荧光酮络合物而导致的2,4-二氯苯基荧光酮的荧光猝灭。在pH 5.2 - 5.9的缓冲溶液中，铁（III）与2,4-二氯苯基荧光酮相互反应形成红色络合物。通过连续变化法和摩尔比法确定其组成为铁（III）：2,4-二氯苯基荧光酮 = 1:4，发现激发波长和发射波长分别为282和560 nm。铁（III）在4 - 24 ng x mL(-1)范围内呈线性关系。该方法简单快速，已应用于中草药和茶叶样品中痕量铁的测定，结果令人满意。