[白细胞介素-2对受辐照肠上皮细胞生长影响的实验研究]
[An experimental study of the effect of IL-2 on the growth of irradiated intestinal epithelial cells].
作者信息
Fu Kai-fei, Peng Rui-yun, Gao Ya-bing, Wang De-wen, Luo Qing-liang, Yang Yi, Dong Bo
机构信息
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Mar;21(2):250-3.
AIM
To observe the effect of ionizing radiation on intestinal epithelial cells 6 (IEC-6) and the effect of IL-2 on the proliferation and recovery of radiation-injured IEC-6 cells.
METHODS
IEC-6 cells exposed to 4, 8 and 12 Gy gamma ray were analyzed with MTT colorimetry, light microscopy, electron microscopy, DNA gel electrophoresis and flow cytometry at 3, 6, 9, 12 hours and 1, 2, 3 days after radiation. Proliferation of 8 Gy gamma ray-irradiated IEC-6 cells which was treated with IL-2 was detected by MTT colorimetry at 3, 6, 9, 12 and 24 hours after irradiation.
RESULTS
Proliferation of IEC-6 decreased with the increased dose of gamma ray radiation. Apoptotic IEC-6 cells increased apparently at 24 hours after radiation with 8.0 Gy gamma ray, IL-2 could enhance the proliferation of irradiated IEC-6 cells (especially at concentration of 1 x 10(5) U/L) in a dose-dependent manner.
CONCLUSION
4-12 Gy of gamma ray radiation can reduce the proliferation and cause apoptosis of IEC-6 cells. IL-2 can accelerate proliferation of irradiated IEC-6 cells and protect IECs from ionization radiation injury.
目的
观察电离辐射对肠上皮细胞6（IEC-6）的影响以及白细胞介素-2（IL-2）对辐射损伤的IEC-6细胞增殖和恢复的影响。
方法
用4、8和12 Gy的γ射线照射IEC-6细胞，分别于照射后3、6、9、12小时以及1、2、3天，采用MTT比色法、光学显微镜、电子显微镜、DNA凝胶电泳和流式细胞术进行分析。用MTT比色法检测照射后3、6、9、12和24小时IL-2处理的8 Gy γ射线照射的IEC-6细胞的增殖情况。
结果
IEC-6细胞的增殖随着γ射线辐射剂量的增加而降低。8.0 Gy γ射线照射后24小时，凋亡的IEC-6细胞明显增加，IL-2能以剂量依赖的方式增强照射后IEC-6细胞的增殖（尤其是浓度为1×10⁵ U/L时）。
结论
4 - 12 Gy的γ射线辐射可降低IEC-6细胞的增殖并导致其凋亡。IL-2可加速照射后IEC-6细胞的增殖并保护肠上皮细胞免受电离辐射损伤。
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