Haruyama Tetsuya, Sakai Tsutomu, Matsuno Kouhei
Department of Biological Functions and Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka 808-0196, Japan.
Biomaterials. 2005 Aug;26(24):4944-7. doi: 10.1016/j.biomaterials.2005.01.027.
The technology of mercaptide self-assembly has previously been used to coat proteins onto metals, but is not practical for many surfaces. In this study, a short peptidic tag was employed for the electrochemical immobilization of proteins on metals (ECtag). A 6-mer histidine (alpha-amino-1H-imidazole-4-propionic acid) homopeptide was employed as an ECtag ligand, which forms coordinate bonds with Ni2+ and other divalent metal ions. Protein A was chosen as a model protein for the immobilization, and was genetically tagged with an ECtag for immobilization onto a Pt electrode surface through the reduction of ECtag:Ni2+ to ECtag:Ni by the electrode potential.
硫醇盐自组装技术此前已用于将蛋白质包覆在金属上，但对许多表面并不实用。在本研究中，一种短肽标签被用于蛋白质在金属上的电化学固定（EC标签）。一种六聚组氨酸（α-氨基-1H-咪唑-4-丙酸）同肽被用作EC标签配体，它与Ni2+和其他二价金属离子形成配位键。选择蛋白A作为固定化的模型蛋白，并通过基因标记一个EC标签，以便通过电极电位将EC标签：Ni2+还原为EC标签：Ni，从而将其固定在铂电极表面。
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