[胃癌细胞系的尿激酶型纤溶酶原激活剂表达及其与腹膜种植的相关性]

[uPA expression of gastric cancer cell lines and its correlation with peritoneal seeding].

作者信息

Ding You-Cheng, Zhu Zheng-Gang, Liu Bing-Ya, Ji Yu-Bao, Chen Xue-Hua, Zhang Yi, Yu Ying-Yan, Lin Yan-Zhen

机构信息

Department of General Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2005 Jan;27(1):13-5.

PMID:15771790

Abstract

OBJECTIVE

To study the correlation between expression of urokinase-type plasminogen activator (uPA) and capability of tumor cell seeding to the peritoneal membrane by different gastric cancer lines.

METHODS

Expression of uPA in 4 human gastric cancer cell lines was examined by semi-quantitative RT-PCR, ELISA and Western blot. uPA activity was determined by an assay kit. After ip inoculation of cancer cells to nude mice, tumors on peritoneal membrane was grossly examined for tumor cell seedings.

RESULTS

SGC7901 was the highest in uPA expression among human gastric cancer cell lines AGS, SGC7901, MKN45, and MKN28. MKN45 had the strongest uPA activity, while AGS was lowest in both uPA expression and activity. Peritoneal seeding tumors of various sizes were observed in mice inoculated with SGC7901 and MKN45 cells. In addition to peritoneal seedings, bloody ascites was present in mice inoculated with MKN28. The MKN45-inoculated mice took the least time to develop tumors and had the shortest surviving period. No peritoneal seeding was seen in mice inoculated with AGS cells.

CONCLUSION

Three of 4 human gastric cancer cell lines studied express uPA mRNA and activity, which correlate with their peritoneal seeding potentials.

摘要

目的

研究尿激酶型纤溶酶原激活剂（uPA）的表达与不同胃癌细胞系肿瘤细胞向腹膜种植能力之间的相关性。

方法

采用半定量逆转录聚合酶链反应（RT-PCR）、酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法检测4种人胃癌细胞系中uPA的表达。用试剂盒测定uPA活性。将癌细胞腹腔接种到裸鼠后，大体检查腹膜上的肿瘤以观察肿瘤细胞种植情况。

结果

在人胃癌细胞系AGS、SGC7901、MKN45和MKN28中，SGC7901的uPA表达最高。MKN45的uPA活性最强，而AGS的uPA表达和活性均最低。接种SGC7901和MKN45细胞的小鼠出现了大小不一的腹膜种植瘤。接种MKN28的小鼠除出现腹膜种植外，还有血性腹水。接种MKN45的小鼠肿瘤形成时间最短，生存期也最短。接种AGS细胞的小鼠未出现腹膜种植。