Suppr超能文献

反义人端粒酶RNA与反义人端粒酶催化亚基联合抑制宫颈癌Hela细胞生长

[Combination of antisense human telomerase RNA and antisense human telomerase catalytic subunit inhibits cervical cancer Hela cells growth].

作者信息

Lei Li-xiu, Chen Zhong-dong

机构信息

Department of Obstetrics and Gynecology, Nanhua University First Hospital, Hengyang 421001, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2005 Jan;40(1):42-6.

Abstract

OBJECTIVE

To investigate the inhibitory effect of combination of antisense human telomerase RNA (anti-hTR) and antisense human telomerase catalytic subunit (anti-hTERT) on proliferation of cervical cancer Hela cell line cultured in vitro.

METHODS

Cervical cancer Hela cells were transfected by anti-hTR, anti-hTERT, anti-hTR + anti-hTERT, sense-hTR, sense-hTERT with Oligofectamin reagent. The proliferation activity of cervical cancer Hela cells was determined using methyl thiazolyl tetrazolium (MTT). The activity of telomerase was tested by polymerase chain reaction-telomeric repeat amplification protocol-enzyme-linked immunosorbent assay (PCR-TRAP-ELISA). Cell morphologies were observed under fluorescence microscope with acridine orange staining. Apoptosis and cell cycle were examined by flow cytometer method (FCM).

RESULTS

The inhibitory rates on proliferation and apoptotic rates of Hela cells transfected by antisense oligonucleotides were significantly higher compared with those of control, sense oligonucleotides and Oligofectamin (all P < 0.01). Meanwhile, the inhibitory rates on proliferation and apoptotic rates of Hela cells transfected by anti-hTR + anti-hTERT were significantly higher compared with those transfected by anti-hTR or anti-hTERT alone (P < 0.01); telomerase activity was significantly decreased (P < 0.01). Transfected by antisense oligonucleotides at 0.2 micromol/L for 3 d, inhibitory rates on proliferation, telomerase activity (relative to control), apoptotic rate of Hela cells was 69.3%, 19.6%, 28.6% respectively (Q = 0.867, 0.919, 1.075). Percentages of G(0)/G(1) phase cells were increased.

CONCLUSIONS

Telomerase anti-hTR and anti-hTERT inhibit Hela cell growth probably through suppressing telomerase activity, inducing apoptosis and blocking cell cycle. Combination of anti-hTR and anti-hTERT has synergistic action on cervical cancer Hela cells.

摘要

目的

探讨反义人端粒酶RNA(anti-hTR)与反义人端粒酶催化亚基(anti-hTERT)联合应用对体外培养的宫颈癌Hela细胞系增殖的抑制作用。

方法

用脂质体转染试剂将anti-hTR、anti-hTERT、anti-hTR + anti-hTERT、正义hTR、正义hTERT转染宫颈癌Hela细胞。采用甲基噻唑基四氮唑(MTT)法检测宫颈癌Hela细胞的增殖活性。通过聚合酶链反应-端粒重复序列扩增协议-酶联免疫吸附测定(PCR-TRAP-ELISA)检测端粒酶活性。用吖啶橙染色在荧光显微镜下观察细胞形态。采用流式细胞仪法(FCM)检测细胞凋亡和细胞周期。

结果

与对照组、正义寡核苷酸组和脂质体转染试剂组相比,反义寡核苷酸转染组对Hela细胞增殖的抑制率和凋亡率显著升高(均P < 0.01)。同时,与单独转染anti-hTR或anti-hTERT相比,anti-hTR + anti-hTERT转染组对Hela细胞增殖的抑制率和凋亡率显著升高(P < 0.01);端粒酶活性显著降低(P < 0.01)。用0.2 μmol/L反义寡核苷酸转染3 d,Hela细胞增殖抑制率、端粒酶活性(相对于对照组)、凋亡率分别为69.3%、19.6%、28.6%(Q = 0.867、0.919、1.075)。G(0)/G(1)期细胞百分比增加。

结论

端粒酶反义hTR和反义hTERT可能通过抑制端粒酶活性、诱导凋亡和阻断细胞周期来抑制Hela细胞生长。anti-hTR与anti-hTERT联合应用对宫颈癌Hela细胞具有协同作用。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验