渗透应激刺激表达鳐鱼（Raja erinacea）AE1的非洲爪蟾卵母细胞中的有机渗透质通道。

Osmotic stress stimulates the organic osmolyte channel in Xenopus laevis oocytes expressing skate (Raja erinacea) AE1.

作者信息

Koomoa Dana-Lynn T, Musch Mark W, Goldstein Leon

机构信息

Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912, USA.

出版信息

J Exp Zool A Comp Exp Biol. 2005 Apr 1;303(4):319-22. doi: 10.1002/jez.a.168.

DOI:10.1002/jez.a.168

PMID:15776417

Abstract

The aim of this study was to determine whether hypo-osmolarity, which activates taurine transport through the volume-sensitive organic osmolyte channel in skate (Raja erinacea) erythrocytes, also activates the organic osmolyte channel activity of skate AE1 (skAE1) expressed in oocytes. When Xenopus laevis oocytes expressing skAE1 were incubated in hypo-osmotic ND 96 (210 mOsm) media, taurine was transported at a significantly higher rate than when incubated in ND 96 (235 mOsm), which is iso-osmotic to Xenopus plasma. Therefore, hypo-osmotic stress is part of the activation mechanism of the organic osmolyte channel in skAE1 expressing oocytes.

摘要

本研究的目的是确定低渗状态是否也能激活在卵母细胞中表达的鳐鱼AE1（skAE1）的有机渗透物通道活性，低渗状态可通过鳐鱼（Raja erinacea）红细胞中的容积敏感性有机渗透物通道激活牛磺酸转运。当将表达skAE1的非洲爪蟾卵母细胞置于低渗的ND 96（210 mOsm）培养基中孵育时，牛磺酸的转运速率显著高于在与非洲爪蟾血浆等渗的ND 96（235 mOsm）中孵育时的转运速率。因此，低渗应激是表达skAE1的卵母细胞中有机渗透物通道激活机制的一部分。