在表达成纤维细胞生长因子受体1（FGF receptor 1）的非洲爪蟾卵母细胞中，ERK2是成纤维细胞生长因子1（FGF1）诱导JNK1磷酸化所必需的。

ERK2 is required for FGF1-induced JNK1 phosphorylation in Xenopus oocyte expressing FGF receptor 1.

作者信息

Browaeys-Poly Edith, Fafeur Véronique, Vilain Jean Pierre, Cailliau Katia

机构信息

Laboratoire de Biologie du Développement, Université des Sciences et Technologies de Lille, UPRES EA 1033, IFR 118, Bâtiment SN3, 59655 Villeneuve D'Ascq Cedex, France.

出版信息

Biochim Biophys Acta. 2005 Mar 22;1743(1-2):1-4. doi: 10.1016/j.bbamcr.2004.07.003.

DOI:10.1016/j.bbamcr.2004.07.003

PMID:15777834

Abstract

A possible connection between the ERK2 and JNK1 MAP kinases transduction cascades was investigated in Xenopus oocytes expressing FGFR1 stimulated by FGF1. Injection of various inhibitors for the Shc/Grb2/Ras/Mos/MEK/ERK2 cascade blocked FGF1-induced germinal vesicle breakdown (GVBD), as well as ERK2 and JNK1 phosphorylation. JNK1 was found to be activated downstream of ERK2, since injection of an active ERK2 triggered JNK1 phosphorylation and inhibition of ERK2 either by a MEK inhibitor or the MKP3 phosphatase blocked JNK1 phosphorylation. These results demonstrated that in FGFR1 signalling JNK1 phosphorylation depends on ERK2.

摘要

在表达受FGF1刺激的FGFR1的非洲爪蟾卵母细胞中，研究了ERK2和JNK1丝裂原活化蛋白激酶（MAP激酶）转导级联之间的可能联系。注射针对Shc/Grb2/Ras/Mos/MEK/ERK2级联的各种抑制剂可阻断FGF1诱导的生发泡破裂（GVBD）以及ERK2和JNK1的磷酸化。发现JNK1在ERK2的下游被激活，因为注射活性ERK2会触发JNK1磷酸化，而通过MEK抑制剂或MKP3磷酸酶抑制ERK2则会阻断JNK1磷酸化。这些结果表明，在FGFR1信号传导中，JNK1磷酸化依赖于ERK2。