大肠杆菌O86 O抗原基因簇的表征及O86特异性基因的鉴定
Characterization of Escherichia coli O86 O-antigen gene cluster and identification of O86-specific genes.
作者信息
Feng Lu, Han Weiqing, Wang Quan, Bastin David A, Wang Lei
机构信息
Teda School of Biological Sciences and Biotechnology, Nankai University, Teda College, Tianjin 300457, China.
出版信息
Vet Microbiol. 2005 Apr 10;106(3-4):241-8. doi: 10.1016/j.vetmic.2004.12.021.
Escherichia coli O86 belongs to the enteropathogenic E. coli (EPEC) group, some strains of which are pathogens of humans, wild birds and farm animals. The O-antigen gene cluster of E. coli O86 was amplified by long-range PCR using primers based on the housekeeping genes galF and gnd, and then sequenced. Genes involved in GDP-Fuc and N-acetyl-galactosamine (GalNAc) synthesis and genes encoding glycosyltransferases, O-unit flippase and O-antigen polymerase were identified on the basis of homology. By screening against 186 E. coli and Shigella-type strains, two genes specific to E. coli O86 were identified. A polymerase chain reaction (PCR) assay, based on the specific O-antigen genes identified here, could be used for the rapid detection of E. coli O86 in environmental and clinical samples. The relationship between E. coli O86 and O127 was also determined by comparing the two O-antigen gene clusters.
大肠杆菌O86属于肠致病性大肠杆菌（EPEC）群，其中一些菌株是人类、野生鸟类和农场动物的病原体。使用基于管家基因galF和gnd的引物，通过长距离PCR扩增大肠杆菌O86的O抗原基因簇，然后进行测序。基于同源性鉴定了参与GDP-岩藻糖和N-乙酰半乳糖胺（GalNAc）合成的基因以及编码糖基转移酶、O单位翻转酶和O抗原聚合酶的基因。通过对186株大肠杆菌和志贺氏菌型菌株进行筛选，鉴定出了两个大肠杆菌O86特有的基因。基于此处鉴定的特异性O抗原基因的聚合酶链反应（PCR）检测方法，可用于快速检测环境和临床样本中的大肠杆菌O86。还通过比较两个O抗原基因簇确定了大肠杆菌O86与O127之间的关系。
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