Shibayama Takao, Masuda Gohta, Ajisawa Atsushi, Hiruma Kiyoshi, Tsuda Fumio, Nishizawa Tsutomu, Takahashi Masaharu, Okamoto Hiroaki
Department of Internal Medicine, Tokyo Metropolitan Komagome Hospital, Tokyo, Japan.
J Med Virol. 2005 May;76(1):24-32. doi: 10.1002/jmv.20319.
To investigate the prevalence of hepatitis B virus (HBV) genotypes and characteristics of HBV isolates among Japanese patients infected with human immunodeficiency virus type 1 (HIV), serum samples collected between September 1990 and March 2002 from 471 HIV-infected patients (age, 38.8 +/- 11.4 [mean +/- standard deviation] years; male, 90%) were tested for hepatitis B surface antigen (HBsAg) and HBV DNA. Positivity for HBsAg and HBV DNA was seen in 42 patients (8.9%), 41 of whom had contracted HIV infection through sexual activity and 1 had hemophilia. Genotypes of HBV were determined by comparative and phylogenetic analyses of the S gene sequence (396 nucleotides [nt]). The distribution of HBV genotypes among the 42 HBV-viremic patients was: A (50%), B (5%), C (24%), D (5%), E (2%), H (10%), A plus D (2%), A plus G (2%). The hemophilia patient had HBV genotype D. Genotypes E, G, and H which had not been reported in Japan, were found in one patient each who had traveled to Zambia, the US, and South America, respectively. Genotypes A and D, which are rare in Japan, were found in patients who had no history of traveling abroad. The entire genome of the HB-JI411 (genotype E [3,212 nt]), HB-JI444G (genotype G [3,248 nt]), and HB-JI260 (genotype H [3,218 nt]) isolates had the highest identity of 98.3%, 99.9%, and 98.5%, respectively, with reported HBV isolates of the same genotype. Most Japanese patients coinfected with HIV and HBV had HBV genotypes that are found rarely or had not been reported in Japan.
为调查1型人类免疫缺陷病毒（HIV）感染的日本患者中乙型肝炎病毒（HBV）基因型的流行情况及HBV分离株的特征，对1990年9月至2002年3月期间收集的471例HIV感染患者（年龄38.8±11.4[均值±标准差]岁；男性占90%）的血清样本进行了乙型肝炎表面抗原（HBsAg）和HBV DNA检测。42例患者（8.9%）HBsAg和HBV DNA呈阳性，其中41例通过性活动感染HIV，1例患有血友病。通过对S基因序列（396个核苷酸[nt]）进行比较和系统发育分析来确定HBV基因型。42例HBV病毒血症患者中HBV基因型的分布为：A（50%）、B（5%）、C（24%）、D（5%）、E（2%）、H（10%）、A+D（2%）、A+G（2%）。该血友病患者的HBV基因型为D。在分别前往赞比亚、美国和南美洲旅行的患者中各发现1例基因型为E、G和H的患者，这几种基因型在日本此前未见报道。在无国外旅行史的患者中发现了在日本较为罕见的基因型A和D。HB-JI411（基因型E[3212 nt]）、HB-JI444G（基因型G[3248 nt]）和HB-JI260（基因型H[3218 nt]）分离株的全基因组与已报道的相同基因型HBV分离株的最高同源性分别为98.3%、99.9%和98.5%。大多数合并感染HIV和HBV的日本患者的HBV基因型在日本很少见或此前未见报道。
