兔和豚鼠输精管对电场刺激的收缩反应的比较研究。

A comparative study on contractile responses of rabbit and guinea pig vasa deferentia to electrical field stimulation.

作者信息

Nakanishi Hironori, Ohkubo Satoko, Ono Tomoyuki, Matsuoka Isao, Nakahata Norimichi

机构信息

Department of Welfare, Fukushima College for Sincerity, Fukushima, Japan.

出版信息

Fukushima J Med Sci. 2004 Dec;50(2):55-64. doi: 10.5387/fms.50.55.

DOI:10.5387/fms.50.55

PMID:15779571

Abstract

Contractile responses of rabbit and guinea pig vasa deferentia to electrical field stimulation (EFS) are compared. A muscarinic receptor blocking agent, 1 microM atropine markedly reduced phasic and tonic contraction induced by EFS (20 Hz, 0.5 msec, 30 V, for 30 sec) in rabbit vas deferens, while it only slightly depressed those in guinea pig vas deferens. Further addition of an adrenergic alpha1 receptor blocking agent, 1 microM prazosin markedly depressed the second tonic contraction in both rabbit and guinea pig vasa deferentia. In the presence of atropine and prazosin, further addition of a P2X purinoceptor desensitizing agent, 10 microM alpha,beta-methylene ATP (alpha, beta-MeATP) abolished the residual phasic contractile response in guinea pig vas deferens, while it partially depressed that in rabbit vas deferens. The administration of 10 microM alpha,beta-MeATP in the absence of atropine and prazosin markedly potentiated the phasic contractile response of rabbit vas deferens to EFS, while it depressed that of guinea pig vas deferens. Contractile response of rabbit vas deferens to alpha,beta-MeATP was more potent than those of ATP and 2-methyl-thioATP (2-Me-thioATP), while these nucleotides had almost same potency in guinea pig vas deferens. These findings may indicate that contribution of cholinergic, adrenergic and purinergic neurotransmission to the contractile response of rabbit vas deferens to EFS is different from that of guinea pig vas deferens.

摘要

比较了兔和豚鼠输精管对电场刺激（EFS）的收缩反应。一种毒蕈碱受体阻断剂，1微摩尔阿托品可显著降低兔输精管中由EFS（20赫兹，0.5毫秒，30伏，持续30秒）诱导的相性和强直性收缩，而对豚鼠输精管中此类收缩的抑制作用则较弱。进一步添加一种肾上腺素能α1受体阻断剂，1微摩尔哌唑嗪可显著抑制兔和豚鼠输精管中的第二次强直性收缩。在阿托品和哌唑嗪存在的情况下，再添加一种P2X嘌呤受体脱敏剂，10微摩尔α,β-亚甲基ATP（α,β-MeATP）可消除豚鼠输精管中残余的相性收缩反应，而对兔输精管中该反应仅有部分抑制作用。在不存在阿托品和哌唑嗪的情况下给予10微摩尔α,β-MeATP可显著增强兔输精管对EFS的相性收缩反应，而对豚鼠输精管的该反应则有抑制作用。兔输精管对α,β-MeATP的收缩反应比对ATP和2-甲基硫代ATP（2-Me-thioATP）的反应更强，而这些核苷酸在豚鼠输精管中的效力几乎相同。这些发现可能表明，胆碱能、肾上腺素能和嘌呤能神经传递对兔输精管对EFS收缩反应的贡献与豚鼠输精管不同。