Mastro A M, Krupa T A, Smith P
Cancer Res. 1979 Oct;39(10):4078-82.
12-O-Tetradecanoylphorbol-13-acetate (TPA) inhibits DNA synthesis in bovine lymph node lymphocytes in mixed-lymphocyte cultures. Using a radioautographic study, we determined that TPA also blocked morphological changes in these cultures. Pretreatment of cultures of isologous lymphocytes with 10(-7) M TPA for three days prior to mixing was sufficient to block their subsequent response in mixed culture. Thus, TPA did not need to be present during the initial cell-to-cell interactions of the mixed lymphocyte response. The inhibition was not due to the death of the responding cell population because the effect was reversible. In one-way mixed-lymphocyte cultures, TPA pretreatment of either responding or stimulating cells could block DNA synthesis. The responding cells were more sensitive to TPA than were the stimulating cells. The inhibitory effect of the stimulators increased with an increase in the stimulating-to-responding cell ratio. In one-way cultures, it was also seen that lymphocytes from different animals varied both in their sensitivity to TPA and in their response to TPA-treated cells from other animals. The data taken together are consistent with the idea that TPA acts by changing cell surface recognition structures and/or indirectly, through activation of a subpopulation of cells to block the proliferative response. TPA may prove to be a valuable tool in studying cell-cell interactions and lymphocyte differentiation in vitro.
12 - 十四酰佛波醇 - 13 - 乙酸酯（TPA）可抑制混合淋巴细胞培养体系中牛淋巴结淋巴细胞的DNA合成。通过放射自显影研究，我们发现TPA还能阻断这些培养体系中的形态学变化。在混合前，用10⁻⁷M TPA对同源淋巴细胞培养物预处理三天，足以阻断其在混合培养中的后续反应。因此，在混合淋巴细胞反应的初始细胞间相互作用过程中，并不需要TPA的存在。这种抑制作用并非由于反应细胞群体的死亡，因为该效应是可逆的。在单向混合淋巴细胞培养体系中，对反应细胞或刺激细胞进行TPA预处理均可阻断DNA合成。反应细胞对TPA比刺激细胞更敏感。刺激细胞的抑制作用随着刺激细胞与反应细胞比例的增加而增强。在单向培养体系中，还发现来自不同动物的淋巴细胞对TPA的敏感性以及对来自其他动物的经TPA处理细胞的反应均有所不同。综合这些数据表明，TPA可能通过改变细胞表面识别结构和/或间接通过激活细胞亚群来阻断增殖反应。TPA可能会被证明是研究体外细胞间相互作用和淋巴细胞分化的一种有价值的工具。
