Chaudhury P, Bera B C
Division of Bacteriology & Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, India.
Int J Immunogenet. 2005 Apr;32(2):75-8. doi: 10.1111/j.1744-313X.2005.00493.x.
Summary Full-length cDNA (582 bp) of the interleukin-18 (IL-18) gene of the Indian water buffalo (Bubalus bubalis) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The deduced amino acid sequence has 99% and 95% similarity with the IL-18 sequences of cattle and sheep, respectively. There are two amino acid substitutions at positions 132 and 182 in buffalo IL-18 compared with that of cattle. Phylogenetic analysis showed that the IL-18 sequence of fish forms a different lineage and is most divergent from that of cattle, buffalo, sheep, pig, dog, horse, human, monkey, mouse, rat and chicken.
摘要 通过逆转录聚合酶链反应（RT-PCR）扩增并测序了印度水牛（Bubalus bubalis）白细胞介素18（IL-18）基因的全长cDNA（582 bp）。推导的氨基酸序列与牛和绵羊的IL-18序列分别具有99%和95%的相似性。与牛相比，水牛IL-18在第132和182位有两个氨基酸替换。系统发育分析表明，鱼类的IL-18序列形成一个不同的谱系，与牛、水牛、绵羊、猪、狗、马、人、猴、小鼠、大鼠和鸡的IL-18序列差异最大。
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