Thériault Jimmy R, Mambula Salamatu S, Sawamura Tatsuya, Stevenson Mary Ann, Calderwood Stuart K
Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 21-27 Burlington Avenue, Boston, MA 02215, USA.
FEBS Lett. 2005 Mar 28;579(9):1951-60. doi: 10.1016/j.febslet.2005.02.046.
Extracellular HSP70 has been found to participate in both innate and adaptive immune responses. However, little is known about the molecular mechanisms that mediate this process. Previous reports suggest that HSP70 interacts with antigen presenting cells (APC) through a plethora of surface receptors. In this study, we have examined the relative binding of potential HSP70 receptors and found high affinity binding to LOX-1 but not other structures with a role in HSP70-APC interactions such as LRP/CD91, CD40, TLR2, TLR4 or another c-type lectin family member (DC-SIGN) closely related to LOX-1. In addition to APC, HSP70 can avidly bind to non-APC cell lines, especially those from epithelial or endothelial background.
已发现细胞外热休克蛋白70（HSP70）参与先天性和适应性免疫反应。然而，介导这一过程的分子机制却鲜为人知。先前的报道表明，HSP70通过大量表面受体与抗原呈递细胞（APC）相互作用。在本研究中，我们检测了潜在HSP70受体的相对结合情况，发现其与凝集素样氧化低密度脂蛋白受体1（LOX-1）有高亲和力结合，但与其他在HSP70-APC相互作用中起作用的结构（如低密度脂蛋白受体相关蛋白/CD91、CD40、Toll样受体2（TLR2）、Toll样受体4（TLR4）或与LOX-1密切相关的另一种C型凝集素家族成员（树突状细胞特异性细胞间黏附分子3抓取非整合素，DC-SIGN））无结合。除APC外，HSP70还能与非APC细胞系紧密结合，尤其是那些来源于上皮或内皮的细胞系。
