Guo Shan-yi, Zuo Ai-jun, Liu Nian-qing, Zhao Xue-qin, Guo Ruo-lin, Zhang Fang, Zhang Jing-yu
Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2005 Jan;39(1):30-2.
To investigate the changing of T4 5'-and 5-deiodinase within rat brain under various iodin-nutritional states.
Animal model of iodine-deficiency rat was performed and the rats were divided into 4 groups by the intake of iodine-nutrition, and then killed at an age of 20 days. The thyroid hormones level in serum was measured by ELISA and the activity of T(4) 5'-and 5-deiodinase within brain was analyzed.
In less-iodine (LI) group,TT4 and FT4 were accounting for 3.5% of the neutral-iodine (NI) group's, and FT3 was 174.0% of NI group's (P < 0.05). In NI group,TT4 and FT4 were 114.5% and 127.7% of NI group's (P < 0.05). In high-iodine (HI) group, TT4 and FT4 were 61.86% and 62.0% of NI group's, and FT3 was 184.9% of NI group's (P < 0.05). In LI group, the activity of T4 5'-deiodinase tissue of per gram (1.95 +/- 0.32) ngT3.microgT4(-1).h was significantly higher than that of NI group (P < 0.05), and the activity of 5-deiodinase (1.38 +/- 0.21) ngrT3.microg T4(-1).h(-1) is significantly less than that of NI group (1.59 +/- 0.23) (P < 0.05). In HI group the activity of T4 5'-and 5-deiodinase tissue of per gram (1.12 +/- 0.19 and 1.73 +/- 0.36) ngrT3.microgT4(-1).h(-1)was significantly less than that of NI group (P < 0.05).
The activity of T4 5'-deiodinase in iodine deficiency heightens and that in iodine excess is debased, the activity of T4 5-deiodinase in iodine deficiency and in iodine excess is debased.
研究不同碘营养状态下大鼠脑内T4 5'-脱碘酶和5-脱碘酶的变化。
建立碘缺乏大鼠动物模型，根据碘营养摄入量将大鼠分为4组，20日龄时处死。采用酶联免疫吸附测定法检测血清甲状腺激素水平，并分析脑内T4 5'-脱碘酶和5-脱碘酶的活性。
低碘（LI）组TT4和FT4占正常碘（NI）组的3.5%，FT3为NI组的174.0%（P<0.05）。正常碘组TT4和FT4分别为NI组的114.5%和127.7%（P<0.05）。高碘（HI）组TT4和FT4分别为NI组的61.86%和62.0%，FT3为NI组的184.9%（P<0.05）。LI组每克组织T4 5'-脱碘酶活性（1.95±0.32）ngT3·μgT4-1·h显著高于NI组（P<0.05），5-脱碘酶活性（1.38±0.21）ngrT3·μg T4-1·h-1显著低于NI组（1.59±0.23）（P<0.05）。HI组每克组织T4 5'-脱碘酶和5-脱碘酶活性（1.12±0.19和1.73±0.36）ngrT3·μgT4-1·h-1显著低于NI组（P<0.05）。
碘缺乏时T4 5'-脱碘酶活性升高，碘过量时降低；碘缺乏和碘过量时T4 5-脱碘酶活性均降低。
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