Chen Jing, Wang Jia-Han, Ren Jia-Liang
Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100041, P.R. China.
Zhonghua Shao Shang Za Zhi. 2005 Feb;21(1):55-6.
To explore the dynamic changes in the expression of c-fos, proliferation cell nuclear antigen (PCNA) and Bax in the intestinal tissue of scalded rats before and after resuscitation.
Wistar rats inflicted with 30% TBSA full thickness scald were employed as the model and randomly divided into four groups with 8 in each group, i.e. 2.0, 2.5, 4.0, 6.0 postscald hour (PSH) groups. Rats in each group received routine fluid infusion at 2 PSH, and were sacrificed at 2, 2.5, 4, 6 PSH, respectively. Then the intestinal tissue of the rats was harvested for the detection of the expression of c-fos, PCNA and Bax.
The expression of c-fos, PCNA and Bax at 2.0 PSH group (65.8 +/- 4.2%, 74.5 +/- 2.4%, 26.3 +/- 5.7%, respectively) significantly increased when compared with those in 2.5 PSH group (92.4 +/- 5.7%, 85.6 +/- 4.5%, 67.1 +/- 6.6%, respectively) (P < 0.01). The expression of 3 genes increased dramatically at 2.5 and 4.0 PSH, and reached the peak at 2.5 PSH. There was no obvious difference in the gene expression between 4 PSH and 2 PSH groups.
The expression of apoptotic genes in the intestinal tissue of scalded rats increased significantly during early resuscitation stage after burn injury.
探讨烫伤大鼠复苏前后肠组织中c-fos、增殖细胞核抗原（PCNA）和Bax表达的动态变化。
采用30%TBSA全层烫伤的Wistar大鼠作为模型，随机分为4组，每组8只，即烫伤后2.0、2.5、4.0、6.0小时组。每组大鼠在烫伤后2小时接受常规液体输注，并分别在烫伤后2、2.5、4、6小时处死。然后采集大鼠肠组织，检测c-fos、PCNA和Bax的表达。
2.0小时组c-fos、PCNA和Bax的表达（分别为65.8±4.2%、74.5±2.4%、26.3±5.7%）与2.5小时组（分别为92.4±5.7%、85.6±4.5%、67.1±6.6%）相比显著升高（P<0.01）。这3种基因的表达在烫伤后2.5和4.0小时显著增加，并在2.5小时达到峰值。4小时组与2小时组的基因表达无明显差异。
烫伤大鼠肠组织中凋亡基因的表达在烧伤后早期复苏阶段显著增加。
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