过程变量对杯芳烃键合固定相上三环类抗精神病药物高效液相色谱分离的影响。
The effect of the process variables on the HPLC separation of tricyclic neuroleptics on a calixarene-bonded stationary phase.
作者信息
Hashem H, Jira T H
机构信息
Institute of Pharmacy, Pharmaceutical/Medicinal Chemistry, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany.
出版信息
Pharmazie. 2005 Mar;60(3):186-92.
The chromatographic behavior of a new HPLC-stationary phase with supramolecular selectors on the basis of calixarenes is described for the separation of nine tricyclic neuroleptics. The effects of different chromatographic conditions (buffer system, pH-value, type and content of organic modifier, injection volume) on the separation of the analytes were studied. Additionally, the effect of structural differences of the neuroleptic analytes was studied. The chemical structure and pKa of the neuroleptics highly influenced their separation on the calix[8]arene phase. The separation of all analytes on the investigated calixarene-bonded stationary phase was possible with a mobile phase of acetonitrile with 30 mM ammonium acetate buffer (pH 3.5) 30:70(v/v) using 1 ml/min flow rate.
描述了一种基于杯芳烃的带有超分子选择剂的新型高效液相色谱固定相用于分离九种三环类抗精神病药物的色谱行为。研究了不同色谱条件（缓冲体系、pH值、有机改性剂的类型和含量、进样体积）对分析物分离的影响。此外，还研究了抗精神病药物分析物结构差异的影响。抗精神病药物的化学结构和pKa对它们在杯[8]芳烃固定相上的分离有很大影响。使用1 ml/min的流速，以乙腈与30 mM醋酸铵缓冲液（pH 3.5）按30:70（v/v）组成的流动相，在所研究的杯芳烃键合固定相上可以实现所有分析物的分离。
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