[肝细胞癌及非肿瘤性肝组织中p21ras、c-myc和p53癌蛋白的免疫组织化学检测]

[Immunohistochemical detection of p21ras, c-myc and p53 oncoproteins in hepatocellular carcinoma and in non-neoplastic liver tissue].

作者信息

Pannain Vera Lucia Nunes, Bottino Adriana Caroli, Santos Raimunda Telma M, Coelho Henrique Sérgio M, Ribeiro-Filho Joaquim, Alves Venâncio A F

机构信息

Departamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro.

出版信息

Arq Gastroenterol. 2004 Oct-Dec;41(4):225-8. doi: 10.1590/s0004-28032004000400005. Epub 2005 Mar 29.

DOI:10.1590/s0004-28032004000400005

PMID:15806265

Abstract

BACKGROUND

Genetic and epigenetic alterations have been described in animal hepatocarcinogenesis models but need to be studied in human being.

AIMS

To assess the immunoreactivity of p21ras, c-myc and p53 oncoproteins in hepatocellular carcinoma and non neoplastic tissue. Association of the immunoreactivity of these markers with histological grades and patterns, hepatitis B and C were additionally studied.

METHODS

Detection of oncoproteins p21ras, c-myc and p53 was performed immunohistochemically in hepatocellular carcinoma (47 cases) and surrounding non neoplastic liver tissue (40 cases).

RESULTS

Oncoproteins p21ras, c-myc and p53 were detected in 44,7%, 53,2% and 36,2% of the hepatocellular carcinoma cases, respectively. The p21ras and c-myc immunoreactivity has shown a significant association. However there was no association of p21ras, c-myc and p53 detection with hepatitis B and C virus infections, histological grades and patterns. The same significant association between p21ras and c-myc was observed in non-neoplastic tissue with cirrhosis when compared with tissue without it. The p53 immunoreactivity was negative in all non-neoplastic liver tissue samples.

CONCLUSIONS

The immunoreactivity detection of p21ras, c-myc and p53 corroborates previous evidence of their detection in hepatocellular carcinoma that suggest the participation of these proteins in human hepatocarcinogenesis. The significant association between p21ras and c-myc oncoproteins in hepatocellular carcinoma and in cirrhosis can point to an interaction between them mainly, in hepatocarcinogenesis that occurs through cirrhosis.

摘要

背景

遗传和表观遗传改变已在动物肝癌发生模型中得到描述，但仍需在人类中进行研究。

目的

评估p21ras、c-myc和p53癌蛋白在肝细胞癌和非肿瘤组织中的免疫反应性。此外，还研究了这些标志物的免疫反应性与组织学分级和模式、乙型和丙型肝炎的相关性。

方法

采用免疫组织化学方法检测47例肝细胞癌和40例周围非肿瘤性肝组织中癌蛋白p21ras、c-myc和p53。

结果

在肝细胞癌病例中，癌蛋白p21ras、c-myc和p53的检测率分别为44.7%、53.2%和36.2%。p21ras和c-myc的免疫反应性显示出显著相关性。然而，p21ras、c-myc和p53的检测与乙型和丙型肝炎病毒感染、组织学分级和模式无关。与无肝硬化的组织相比，在有肝硬化的非肿瘤组织中也观察到p21ras和c-myc之间存在相同的显著相关性。所有非肿瘤性肝组织样本中p53的免疫反应性均为阴性。