[冰片对大鼠下丘脑透明质酸和5-羟色胺水平的影响]

[The effect of borneol on level of HA and 5-HT in rat's hypothalamus].

作者信息

Li Weirong, Yao Limei, Mi Suiqing, Wang Ningsheng

机构信息

Institute of Clinical Pharmacology, Guangzhou University of TCM, Guangzhou.

出版信息

Zhong Yao Cai. 2004 Dec;27(12):937-9.

PMID:15807248

Abstract

OBJECTIVE

To observe the effect of borneol on level of HA and 5-HT in rat's hypothalamus.

METHODS

The levels of HA and 5-HT in rat's hypothalamus were determined before and after p.o. with different amount of borneol by high performance liquid chromatography with electrochemical detection (HPLC-ECD), and the borneol's effect on the levels of HA and 5-HT was also studied.

RESULTS

The level of HA in rat's hypothalamus after different dose of borneol were higher than before administration. The level of HA in 20 min group after middle dose increased significantly comparing with before administration (P < 0.01), the others of group after middle dose, the 45 min group after high dose, the 20 and 45 min after low dose were also increased significantly than before administration (P < 0.05). After different dose of borneol, the level of 5-HT in rat's hypothalamus changed as follows: the level of 5-HT after high dose were higher than before administraton (P <0.05 or P < 0.01 ); the level of 5-HT after 5, 20 and 45 min of middle and low dose incrased significantly (P <0.05 or P < 0.01).

CONCLUSION

Borneol could increase the levels of HA and 5-HT in rat's hypothalamus.

摘要

目的

观察冰片对大鼠下丘脑组织中透明质酸（HA）和5-羟色胺（5-HT）含量的影响。

方法

采用高效液相色谱-电化学检测法（HPLC-ECD），测定不同剂量冰片灌胃前后大鼠下丘脑组织中HA和5-HT的含量，并研究冰片对其含量的影响。

结果

不同剂量冰片灌胃后，大鼠下丘脑组织中HA含量均高于给药前。中剂量组20分钟时HA含量较给药前显著升高（P<0.01），中剂量组其他时间点、高剂量组45分钟时、低剂量组20分钟和45分钟时HA含量较给药前均显著升高（P<0.05）。不同剂量冰片灌胃后，大鼠下丘脑组织中5-HT含量变化如下：高剂量组5-HT含量高于给药前（P<0.05或P<0.01）；中、低剂量组5、20和45分钟时5-HT含量显著升高（P<0.05或P<0.01）。