一种7872 cDNA微阵列及其在牛功能基因组学中的应用。
A 7872 cDNA microarray and its use in bovine functional genomics.
作者信息
Everts Robin E, Band Mark R, Liu Z Lewis, Kumar Charu G, Liu Lei, Loor Juan J, Oliveira Rosane, Lewin Harris A
机构信息
Department of Animal Sciences, University of Illinois, 206 Edward R. Madigan Laboratory, 1201 West Gregory Drive, Urbana, IL 61801, USA.
出版信息
Vet Immunol Immunopathol. 2005 May 15;105(3-4):235-45. doi: 10.1016/j.vetimm.2005.02.003.
The strategy used to create and annotate a 7872 cDNA microarray from cattle placenta and spleen cDNA sequences is described. This microarray contains approximately 6300 unique genes, as determined by BLASTN and TBLASTX similarity search against the human and mouse UniGene and draft human genome sequence databases (build 34). Sequences on the array were annotated with gene ontology (GO) terms, thereby facilitating data analysis and interpretation. A total of 3244 genes were annotated with GO terms. The array is rich in sequences encoding transcription factors, signal transducers and cell cycle regulators. Current research being conducted with this array is described, and an overview of planned improvements in our microarray platform for cattle functional genomics is presented.
本文描述了用于从牛胎盘和脾脏cDNA序列创建并注释一个7872个cDNA微阵列的策略。通过针对人类和小鼠的UniGene以及人类基因组序列数据库草图（第34版）进行BLASTN和TBLASTX相似性搜索确定，该微阵列包含约6300个独特基因。阵列上的序列用基因本体（GO）术语进行了注释，从而便于数据分析和解读。共有3244个基因用GO术语进行了注释。该阵列富含编码转录因子、信号转导子和细胞周期调节因子的序列。文中描述了目前使用该阵列进行的研究，并概述了我们用于牛功能基因组学的微阵列平台计划中的改进。
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