Maruyama Hiroki, Higuchi Noboru, Kameda Shigemi, Nakamura Gen, Shimotori Masaaki, Iino Noriaki, Higuchi Masato, Neichi Tomohiro, Yokoyama Sadaaki, Kono Toru, Miyazaki Jun-ichi, Gejyo Fumitake
Division of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-dori, Niigata 951-8120.
J Biochem. 2005 Mar;137(3):373-80. doi: 10.1093/jb/mvi038.
Recently, we developed a kidney-targeted gene transfer technique, in which naked DNA was injected into the renal vein while the renal vein and artery were clamped. Kidney-targeted DNA transfer with only the renal vein clamped is an important modification that may permit less invasive catheter-based gene transfer in future clinical applications. The preparation of PCR-amplified DNA fragments is less time-consuming than that of naked plasmid DNA. We examined rat erythropoietin (Epo) plasmid, pCAGGS-Epo, or PCR-amplified DNA fragment, fCAGGS-Epo, transfer into the rat kidney with only the renal vein clamped. The Epo level peaked at week 3 and then was sustained for 24 weeks, which resulted in significant erythropoiesis. This modified technique, allowing long-term expression of both PCR-amplified DNA fragments and naked plasmid DNA, could potentially be used for catheter-based gene transfer in humans, and could help determine the physiological functions of putative genes.
最近，我们开发了一种肾脏靶向基因转移技术，即在夹闭肾静脉和肾动脉的同时将裸DNA注入肾静脉。仅夹闭肾静脉的肾脏靶向DNA转移是一项重要的改进，这可能使未来临床应用中基于导管的基因转移侵入性更小。PCR扩增的DNA片段的制备比裸质粒DNA耗时更少。我们研究了仅夹闭肾静脉时大鼠促红细胞生成素（Epo）质粒pCAGGS-Epo或PCR扩增的DNA片段fCAGGS-Epo向大鼠肾脏的转移。Epo水平在第3周达到峰值，然后持续24周，这导致了显著的红细胞生成。这种改进的技术允许PCR扩增的DNA片段和裸质粒DNA长期表达，可能用于人类基于导管的基因转移，并有助于确定推定基因的生理功能。
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