Fukushima Atsuki, Ozaki Akemi, Jian Zhang, Ishida Waka, Fukata Kazuyo, Ueno Hisayuki, Liu Fu-Tong
Department of Ophthalmology, Kochi Medical School, Kochi University, Nankoku-city 783-8505, Japan.
Curr Eye Res. 2005 Apr;30(4):241-8. doi: 10.1080/02713680590927560.
Allergic conjunctivitis is characterized by allergen-specific IgE in the serum and infiltration of eosinophils into the conjunctiva. However, it remains unclear whether early-phase reaction (EPR) mediated by Ag-specific IgE links to late-phase reaction (LPR) in the conjunctiva. We aimed to investigate whether LPR is mediated by either cellular or humoral immune responses.
Experimental immune-mediated blepharoconjunctivitis (EC) was induced in C57BL/6 mice by either active immunization or passive immunization by transfer of ragweed (RW)-primed lymphocytes and RW-specific IgE, followed by RW challenge onto the conjunctiva. Transferring RW-primed lymphocytes were prepared from RW-primed splenocytes which were stimulated in vitro with RW for 3 days. Fifteen minutes after RW challenge, clinical findings were evaluated and 24 hr after challenge, the conjunctivas and sera were harvested for histologic analysis and measurement of IgE, respectively.
EPR was most prominent when EC was induced by transfer of RW-specific IgE. EPR was hardly detectable if EC was induced by transfer of RW-primed lymphocytes. Mild EPR was noted when EC was induced by active immunization. LPR, evaluated by infiltration of eosinophils into the conjunctiva, was most severe when EC was induced by transfer of RW-primed lymphocytes. Minimal, but definite LPR was induced when EC was induced by transfer of RW-specific IgE. Intermediate severity of LPR was induced when EC was induced by active immunization.
LPR in the conjunctiva is dominantly mediated by cellular immune responses, whereas EPR in the conjunctiva is putatively mediated by humoral immune responses. Importantly, LPR in the conjunctiva is inducible by Ag-specific IgE alone, although minute.
过敏性结膜炎的特征是血清中存在变应原特异性IgE以及嗜酸性粒细胞浸润结膜。然而，尚不清楚由抗原特异性IgE介导的早期反应（EPR）是否与结膜中的晚期反应（LPR）相关。我们旨在研究LPR是否由细胞免疫或体液免疫反应介导。
通过主动免疫或被动免疫，即转移经豚草（RW）致敏的淋巴细胞和RW特异性IgE，随后将RW攻击结膜，在C57BL/6小鼠中诱导实验性免疫介导的睑结膜炎（EC）。转移的经RW致敏的淋巴细胞由经RW体外刺激3天的致敏脾细胞制备。RW攻击后15分钟，评估临床症状，攻击后24小时，分别采集结膜和血清进行组织学分析和IgE测定。
当通过转移RW特异性IgE诱导EC时，EPR最为明显。如果通过转移经RW致敏的淋巴细胞诱导EC，则几乎检测不到EPR。通过主动免疫诱导EC时，可观察到轻度EPR。通过结膜中嗜酸性粒细胞浸润评估的LPR，在通过转移经RW致敏的淋巴细胞诱导EC时最为严重。通过转移RW特异性IgE诱导EC时，诱导出最小但明确的LPR。通过主动免疫诱导EC时，诱导出中等严重程度的LPR。
结膜中的LPR主要由细胞免疫反应介导，而结膜中的EPR推测由体液免疫反应介导。重要的是，结膜中的LPR仅由抗原特异性IgE即可诱导，尽管程度轻微。
