Abir Ronit, Ao Asangla, Jin Shaoguang, Barnett Miriam, Van den Hurk Robert, Freimann Sarit, Fisch Benjamin
Infertility and IVF Unit, Department of Obstetrics and Gynecology, Rabin Medical Center, Beilinson Campus, Petah Tikva 49100, Israel.
Fertil Steril. 2005 Apr;83 Suppl 1:1188-96. doi: 10.1016/j.fertnstert.2004.10.043.
To investigate the immunocytochemical expression and presence of mRNA transcripts of oncostatin M (OSM) and its exclusive receptor (OSM-Rbeta) in ovaries from human adults and fetuses.
Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study.
Major tertiary care and referral academic centers.
PATIENT(S): Ten women and girls undergoing laparoscopic ovarian biopsy and 30 women undergoing second-trimester and third-trimester pregnancy terminations.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Microscopic morphometric analysis, immunocytochemistry for OSM and OSM-Rbeta, and RT-PCR analyses.
RESULT(S): There was strong to moderate immunocytochemical staining for OSM in both oocytes and granulosa cells of follicles from primordial stages onwards in ovaries from both fetuses and adults/adolescents. OSM-Rbeta was detected mainly in the oocytes. Transcripts of OSM and OSM-Rbeta RNA were detected by RT-PCR analyses.
CONCLUSION(S): The expression of OSM and its receptor in ovarian tissue from fetuses and women suggests a possible role of OSM in growth initiation of human primordial follicles.
研究抑瘤素M（OSM）及其特异性受体（OSM-Rβ）在成年和胎儿卵巢中的免疫细胞化学表达及mRNA转录本的存在情况。
免疫细胞化学和逆转录聚合酶链反应（RT-PCR）研究。
主要的三级医疗和转诊学术中心。
10名接受腹腔镜卵巢活检的女性和女孩，以及30名接受孕中期和孕晚期终止妊娠的女性。
无。
显微镜形态计量分析、OSM和OSM-Rβ的免疫细胞化学检测以及RT-PCR分析。
在胎儿和成年/青少年卵巢中，从原始卵泡阶段开始，卵泡的卵母细胞和颗粒细胞中均有强至中度的OSM免疫细胞化学染色。OSM-Rβ主要在卵母细胞中检测到。通过RT-PCR分析检测到了OSM和OSM-Rβ RNA的转录本。
OSM及其受体在胎儿和女性卵巢组织中的表达表明，OSM在人类原始卵泡的生长启动中可能发挥作用。
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