微电子微芯片的分子诊断

Molecular diagnostics by microelectronic microchips.

作者信息

Ferrari Maurizio, Cremonesi Laura, Bonini Pierangelo, Stenirri Stefania, Foglieni Barbara

机构信息

IRCCS Ospedale San Raffaele, Head of the Clinical Molecular Biology & Cytogenetics Laboratory, Diagnostica e Ricerca San Raffaele SPA, and Unit of Genomics for Diagnosis of Human Pathologies, via Olgettina 60, 20132 Milan, Italy.

出版信息

Expert Rev Mol Diagn. 2005 Mar;5(2):183-92. doi: 10.1586/14737159.5.2.183.

DOI:10.1586/14737159.5.2.183

PMID:15833048

Abstract

Molecular diagnostics is being revolutionized by the development of highly advanced technologies for DNA and RNA testing. One of the most important challenges is the integration of microelectronics to microchip-based nucleic acid technologies. The specific characteristics of these microsystems make the miniaturization and automation of any step of a molecular diagnostic procedure possible. This review describes the application of microelectronics to all the processes involved in a genetic test, particularly to sample preparation, DNA amplification and sequence variation detection.

摘要

高度先进的DNA和RNA检测技术的发展正在彻底改变分子诊断学。最重要的挑战之一是将微电子技术与基于微芯片的核酸技术相结合。这些微系统的特殊特性使分子诊断程序的任何步骤的小型化和自动化成为可能。本综述描述了微电子技术在基因检测所涉及的所有过程中的应用，特别是在样品制备、DNA扩增和序列变异检测方面的应用。