[血小板源性生长因子B基因表达对组织工程皮肤移植后血管重建的影响]

[Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting].

作者信息

Tan Qian, Chen Xi, Liang Zhi-wei, Huang Pei-lin, Zhou Hong-reng, Yang Ding-wen, Lin Zi-hao, Jiang Hua

机构信息

Department of Burns and Plastic Surgery, Drum Tower Hospital, Medical College of Nanjing University, Nanjing, China.

出版信息

Zhonghua Zheng Xing Wai Ke Za Zhi. 2004 Nov;20(6):447-50.

PMID:15835805

Abstract

OBJECTIVE

To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats.

METHODS

The recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes + acellular dermal matrix (group A), keratinocytes + acellular dermal matrix + fibroblasts (group B), keratinocytes + acellular dermal matrix + fibroblasts with PDGF gene (group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation.

RESULTS

In 2-4 weeks after skin grafting the vascularization rate in group C was higher than that of group B and group A. The vascularization rates in all groups had no significant differences in six weeks (P > 0.05).

CONCLUSION

PDGF-B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue-engineering skin grafting, which ensures the take of grafted tissue-engineering skin.

摘要

目的

通过将含血小板衍生生长因子-B（PDGF-B）基因的组织工程皮肤移植到大鼠身上，研究PDGF对真皮血管重建的影响。

方法

构建重组真核表达载体pcDNA3.1-hPDGF-B，并通过脂质体介导转染至成纤维细胞。分别将角质形成细胞+无细胞真皮基质（A组）、角质形成细胞+无细胞真皮基质+成纤维细胞（B组）、角质形成细胞+无细胞真皮基质+携带PDGF基因的成纤维细胞（C组）进行重组，然后移植到大鼠背部，术后2、4、6周评估真皮内血管的重建情况。