George M R, John K R, Iyappan T, Jeyaseelan M J P
Department of Aquaculture, Fisheries College and Research Institute, Tuticorin, India.
Lett Appl Microbiol. 2005;40(5):369-72. doi: 10.1111/j.1472-765X.2005.01675.x.
To study the strain variability among Vibrio alginolyticus isolates from different sources by insertion sequence-targeted PCR fingerprinting and whole cell protein profile analysis.
Eleven strains of V. alginolyticus were isolated from seven different sources including healthy, infected, farm-reared and wild shrimps. Following biochemical characterization, the isolates were analysed by PCR fingerprinting and whole cell protein analysis by SDS-PAGE. The strains were genetically different irrespective of the sources of isolation.
Strain variation exists in V. alginolyticus isolates obtained even from the same source, and PCR fingerprinting is a simple and efficient method in identifying strain-specific variations among the different isolates.
Vibrio alginolyticus is implicated in severe vibriosis of marine aquaculture systems although many strains are avirulent and could be used as probiotic strains. As a wide variation exists among this species, differentiating the harmful and beneficial strains would help in finding ways of controlling the infections by eliminating harmful shrimp pathogenic vibrios.
通过插入序列靶向PCR指纹图谱和全细胞蛋白谱分析，研究不同来源的溶藻弧菌分离株之间的菌株变异性。
从包括健康、感染、养殖和野生虾类在内的七个不同来源分离出11株溶藻弧菌。经过生化特性鉴定后，通过PCR指纹图谱和SDS-PAGE全细胞蛋白分析对分离株进行分析。无论分离来源如何，这些菌株在基因上都存在差异。
即使是从同一来源获得的溶藻弧菌分离株也存在菌株变异，PCR指纹图谱是鉴定不同分离株之间菌株特异性变异的一种简单有效的方法。
尽管许多溶藻弧菌菌株无毒且可作为益生菌菌株，但溶藻弧菌与海水养殖系统的严重弧菌病有关。由于该物种存在广泛变异，区分有害和有益菌株将有助于通过消除有害的虾致病弧菌来找到控制感染的方法。
