汞、铬和锰化合物雌激素样作用的实验研究
[Experiment study on the estrogen-like effect of compounds of mercury, chromium and manganese].
作者信息
Wang Ya-Dong, Chen Xiao-Yu, Wu Yi-Ming, Xu Dong
机构信息
School of Public Health, Zhengzhou University, Zhengzhou 450052, China.
出版信息
Wei Sheng Yan Jiu. 2005 Jan;34(1):49-51.
OBJECTIVE
In order to study estrogen-like effect of compounds of Mercury, Chromium and Manganese.
METHODS
Mercury Chloride, Manganese Sulfate, Chromium Chloride and Chromium trioxide were selected to perform proliferation assay of MCF-7 human breast cancer cells and binding assay of estrogen receptor for rat uterine.
RESULTS
Mercury Chloride could stimulate the proliferation of MCF-7 cell, the maximal increase of MCF-7 cells was measured by Mercury Chloride at 10(-7) mol/L. The effect was blocked completely by a pure antigestrogen ICI182, 780; but Mercury Chloride could not bind to ER by competing with E2. Chromium Chloride, Chromium Trioxide and Manganese Sulfate were not able to stimulate the proliferation of MCF-7 cell and bind to ER through competing against 3H-E2 in vitro.
CONCLUSION
Mercury Chloride exhibited the estrogenicity through binding and activating ER; Chromium Chloride, Chromium trioxide and Manganese Sulfate did not show any estrogenicity.
目的
研究汞、铬、锰化合物的雌激素样作用。
方法
选用氯化汞、硫酸锰、氯化铬和三氧化铬进行MCF-7人乳腺癌细胞增殖试验及大鼠子宫雌激素受体结合试验。
结果
氯化汞可刺激MCF-7细胞增殖，10(-7)mol/L氯化汞使MCF-7细胞增殖增加最为明显。该作用可被纯抗雌激素ICI182,780完全阻断；但氯化汞不能通过与E2竞争结合雌激素受体。氯化铬、三氧化铬和硫酸锰在体外不能刺激MCF-7细胞增殖，也不能通过与3H-E2竞争结合雌激素受体。
结论
氯化汞通过结合并激活雌激素受体表现出雌激素活性；氯化铬、三氧化铬和硫酸锰未表现出任何雌激素活性。
Zotero 插件