扇贝二甲基色胺作为一种钙离子转运体发挥作用。

Scallop DMT functions as a Ca2+ transporter.

作者信息

Toyohara Haruhiko, Yamamoto Sayuri, Hosoi Masatomi, Takagi Masaya, Hayashi Isao, Nakao Kenji, Kaneko Shuji

机构信息

Laboratory of Marine Biological Function, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan.

出版信息

FEBS Lett. 2005 May 9;579(12):2727-30. doi: 10.1016/j.febslet.2005.03.082. Epub 2005 Apr 9.

DOI:10.1016/j.febslet.2005.03.082

PMID:15862316

Abstract

We identified a DMT (divalent metal transporter) homologous protein that functions as a Ca(2+) transporter. Scallop DMT cDNA encodes a 539-amino-acid protein with 12 putative membrane-spanning domains and has a consensus transport motif in the fourth extracellular loop. Since its mRNA is significantly expressed in the gill and intestine, it is assumed that scallop DMT transports Ca(2+) from seawater by the gill and from food by the intestine. Scallop DMT lacks the iron-responsive element commonly found in iron-regulatory proteins, suggesting that it is free of the post-transcriptional regulation from intracellular Fe(2+) concentration. Scallop DMT distinctly functions as a Ca(2+) transporter unlike other DMTs, however, it also transports Fe(2+) and Cd(2+) similar to them.

摘要

我们鉴定出一种作为Ca(2+)转运蛋白发挥作用的二价金属转运体（DMT）同源蛋白。扇贝DMT cDNA编码一个含有12个推定跨膜结构域的539个氨基酸的蛋白，并且在第四个细胞外环中有一个共有转运基序。由于其mRNA在鳃和肠道中显著表达，推测扇贝DMT通过鳃从海水中转运Ca(2+)，并通过肠道从食物中转运Ca(2+)。扇贝DMT缺乏铁调节蛋白中常见的铁反应元件，这表明它不受细胞内Fe(2+)浓度的转录后调控。与其他DMT不同，扇贝DMT明显作为一种Ca(2+)转运蛋白发挥作用，然而，它也与其他DMT类似地转运Fe(2+)和Cd(2+)。