辐射对人结肠癌细胞黏附分子的体外生物学效应。
Biological effect of irradiation on adhesion molecules in human colon cancer cells in vitro.
作者信息
Hasegawa Takeshi, Sato Mikinori, Kurimoto Masaaki, Takahashi Hiroki, Kawashima Takashi, Matsuo Youichi, Yamamoto Minoru, Sawai Hirozumi, Funahashi Hitoshi, Okada Yuji, Takeyama Hiromitsu, Manabe Tadao
机构信息
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
出版信息
Anticancer Res. 2005 Mar-Apr;25(2A):875-9.
BACKGROUND
Carbohydrate antigens, such as sialyl Lewis(a) antigen (s-Le(a)) and sialyl Lewis(x) antigen (s-Le(x)), play an important role in cancer metastasis in vitro and in vivo. Currently, preoperative radiotherapy is used to prevent local recurrence of rectal cancer. We investigated the effects of X-ray irradiation on the carbohydrate antigens s-Le(a) and s-Le(x) in vitro.
MATERIALS AND METHODS
The cell surface expressions of s-Le(a) and s-Le(x) were determined by flow cytometric analysis at 24 hours after X-ray irradiation of 4 human cancer cell lines. s-Le(a) and s-Le(x) functions were quantitated using a monolayer cell adhesion assay with human umbilical vein endothelial cells (HUVECs).
RESULTS
The cell surface expressions of s-Le(a) and s-Le(x) decreased at 24 hours after irradiation. s-Le(a) adhesion to HUVECs monolayers similarly decreased at 24 hours after irradiation.
CONCLUSION
These results may indicate a role for X-ray irradiation in the reduction of liver metastasis in patients with colon cancer.
背景
碳水化合物抗原，如唾液酸化路易斯（a）抗原（s-Le(a)）和唾液酸化路易斯（x）抗原（s-Le(x)），在体外和体内的癌症转移中发挥重要作用。目前，术前放疗用于预防直肠癌的局部复发。我们在体外研究了X射线照射对碳水化合物抗原s-Le(a)和s-Le(x)的影响。
材料与方法
对4种人类癌细胞系进行X射线照射后24小时，通过流式细胞术分析测定s-Le(a)和s-Le(x)的细胞表面表达。使用人脐静脉内皮细胞（HUVECs）单层细胞黏附试验对s-Le(a)和s-Le(x)的功能进行定量。
结果
照射后24小时，s-Le(a)和s-Le(x)的细胞表面表达降低。照射后24小时，s-Le(a)对HUVECs单层细胞的黏附同样降低。
结论
这些结果可能表明X射线照射在降低结肠癌患者肝转移方面发挥作用。
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