Ault Kevin A, Allen Heather K, Phillips Stacia L, Zimmerman M Bridget, Klingelhutz Aloysius J
Department of Obstetrics and Gynecology, University of Iowa, Iowa City, Iowa, USA.
J Low Genit Tract Dis. 2005 Apr;9(2):93-9. doi: 10.1097/00128360-200504000-00005.
To determine whether there is an association between high levels of telomerase and premalignant cervical disease and to provide a preliminary analysis of telomerase activity as a potential triage strategy.
Premenopausal women were invited to participate in the study during routine gynecologic visits as well as visits where colposcopy was performed. Samples were taken from the cervix using a broom device and placed in cold phosphate-buffered saline. A total of 92 samples were evaluated. Cells were counted and lysed, and a semiquantitative measure of telomerase activity was determined using a commercially available telomerase enzyme-linked immunosorbent assay kit. The presence of human papillomavirus (HPV) types 16 and 18 was assessed by polymerase chain reaction analysis. One-way analysis of variance was used to test for the association of telomerase activity with cytology, HPV type 16 or 18 status, and colposcopy and/or biopsy findings.
When telomerase levels were analyzed according to Pap smear results, there were no differences among four groups of cytology findings (normal, atypical squamous cells of undetermined significance, low-grade squamous intraepithelial lesion, and high-grade squamous intraepithelial lesion). When colposcopy and/or biopsy results were considered, significantly higher levels of telomerase were detected in cervical intraepithelial neoplasia (CIN) 2,3 samples than in normal Pap smear samples and CIN 1 samples (p = .035). There was no significant difference in telomerase levels between samples that tested positive for HPV type 16 or 18 and those that did not (p = .111).
Telomerase levels were significantly higher in cytologic samples from women with biopsy-proven CIN 2,3 than in samples from women with normal cytology results or CIN 1. These results warrant larger studies to determine whether telomerase activity may be a useful triage tool for abnormal cytologic findings.
确定端粒酶水平升高与癌前宫颈疾病之间是否存在关联，并对端粒酶活性作为一种潜在的分流策略进行初步分析。
邀请绝经前女性在常规妇科检查以及进行阴道镜检查时参与本研究。使用扫帚状装置从宫颈采集样本，并置于冷的磷酸盐缓冲盐水中。共评估了92个样本。对细胞进行计数并裂解，使用市售的端粒酶酶联免疫吸附测定试剂盒测定端粒酶活性的半定量指标。通过聚合酶链反应分析评估16型和18型人乳头瘤病毒（HPV）的存在情况。采用单因素方差分析来检验端粒酶活性与细胞学、HPV 16或18型状态以及阴道镜检查和/或活检结果之间的关联。
根据巴氏涂片结果分析端粒酶水平时，四组细胞学结果（正常、意义不明确的非典型鳞状细胞、低级别鳞状上皮内病变和高级别鳞状上皮内病变）之间没有差异。当考虑阴道镜检查和/或活检结果时，在宫颈上皮内瘤变（CIN）2、3级样本中检测到的端粒酶水平明显高于正常巴氏涂片样本和CIN 1级样本（p = 0.035）。HPV 16或18型检测呈阳性的样本与未呈阳性样本的端粒酶水平没有显著差异（p = 0.111）。
活检证实为CIN 2、3级的女性细胞学样本中的端粒酶水平明显高于细胞学结果正常或CIN 1级女性的样本。这些结果需要更大规模的研究来确定端粒酶活性是否可能是异常细胞学结果有用的分流工具。
