嗜肺军团菌费城-1型的tatB和tatC影响细胞内复制和生物膜形成。
Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation.
作者信息
De Buck Emmy, Maes Liesbeth, Meyen Eef, Van Mellaert Lieve, Geukens Nick, Anné Jozef, Lammertyn Elke
机构信息
Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Louvain, Belgium.
出版信息
Biochem Biophys Res Commun. 2005 Jun 17;331(4):1413-20. doi: 10.1016/j.bbrc.2005.04.060.
Legionella pneumophila is a facultative intracellular human pathogen and an important cause of Legionnaires' disease, a severe form of pneumonia. Recently, we showed the presence of a putative twin-arginine translocation (Tat) pathway in L. pneumophila Philadelphia-1. This secretion pathway is used to transport completely folded proteins across the cytoplasmic membrane. The importance of the Tat pathway in L. pneumophila was investigated by constructing a tatB and a tatC mutant. Functionality of the Tat pathway was shown using a proven heterologous Tat substrate. It was shown that tatB and tatC are involved in intracellular replication in Acanthamoeba castellanii and differentiated U937 cells, and in biofilm forming ability. A putative Legionella Tat substrate was identified via 2D gel electrophoresis.
嗜肺军团菌是一种兼性胞内人类病原体，也是军团病（一种严重的肺炎形式）的重要病因。最近，我们发现嗜肺军团菌费城-1型中存在一种假定的双精氨酸转运（Tat）途径。该分泌途径用于将完全折叠的蛋白质转运穿过细胞质膜。通过构建tatB和tatC突变体来研究Tat途径在嗜肺军团菌中的重要性。使用经过验证的异源Tat底物来证明Tat途径的功能。结果表明，tatB和tatC参与了嗜肺军团菌在卡氏棘阿米巴和分化的U937细胞中的胞内复制以及生物膜形成能力。通过二维凝胶电泳鉴定了一种假定的军团菌Tat底物。
Zotero 插件