人抗狂犬病免疫球蛋白在聚苯乙烯表面的被动吸附。

Passive adsorption of human antirrabic immunoglobulin onto a polystyrene surface.

作者信息

Rosado E, Caroll H, Sánchez O, Peniche C

机构信息

Department of Macromolecular Chemistry, Biomaterials Center BIOMAT, University of Havana, P.O. Box 6130, Postal Code 10600, Havana, Cuba.

出版信息

J Biomater Sci Polym Ed. 2005;16(4):435-48. doi: 10.1163/1568562053700165.

DOI:10.1163/1568562053700165

PMID:15887652

Abstract

The latex agglutination immunoassay technique uses polymer colloids as carriers of adsorbed proteins to enhance the antigen-antibody reaction. The aim of the present work is to study the adsorption of Human Antirrabic Immunoglobulin (HA-IgG) on polystyrene latex (PS). The physical adsorption of HA-IgG on PS latex was investigated as a function of pH at 2 mM ionic strength. The amount of HA-IgG adsorbed onto PS latex greatly depends on pH; its value showed a maximum in the neighborhood of the IEP of HA-IgG. The electrophoretic method was applied to characterize latex particles. The influence of the amount of HA-IgG adsorbed (J(ads)) on the electrophoretic mobility and zeta-potential values was also studied.

摘要

乳胶凝集免疫测定技术使用聚合物胶体作为吸附蛋白质的载体，以增强抗原 - 抗体反应。本研究的目的是研究人抗狂犬病免疫球蛋白（HA-IgG）在聚苯乙烯乳胶（PS）上的吸附情况。在2 mM离子强度下，研究了HA-IgG在PS乳胶上的物理吸附与pH的关系。吸附到PS乳胶上的HA-IgG量很大程度上取决于pH；其值在HA-IgG的等电点附近出现最大值。采用电泳方法对乳胶颗粒进行表征。还研究了吸附的HA-IgG量（J(ads)）对电泳迁移率和zeta电位值的影响。