日本囊对虾Y器官中蜕皮抑制激素（MIH）受体的特性研究

Characterization of a molt-inhibiting hormone (MIH) receptor in the Y-organ of the kuruma prawn, Marsupenaeus japonicus.

作者信息

Asazuma Hideaki, Nagata Shinji, Katayama Hidekazu, Ohira Tsuyoshi, Nagasawa Hiromichi

机构信息

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo, Japan.

出版信息

Ann N Y Acad Sci. 2005 Apr;1040:215-8. doi: 10.1196/annals.1327.027.

DOI:10.1196/annals.1327.027

PMID:15891027

Abstract

To characterize a receptor for molt-inhibiting hormone (MIH) in the kuruma prawn, Marsupenaeus japonicus, we performed a binding assay and chemical cross-linking experiments using radiolabeled recombinant MIH ([(125)I]rMIH). The specific binding of [(125)I]rMIH was found in the membrane fraction of the Y-organ (K(d) = 4.76 x 10(-10) M and B(max) = 5.51 x 10(-12) M). Chemical cross-linking between [(125)I]rMIH and the membrane fraction of the Y-organ revealed that the MIH receptor protein has a molecular size of approximately 70 kDa.

摘要

为了鉴定日本对虾（Marsupenaeus japonicus）中蜕皮抑制激素（MIH）的受体，我们使用放射性标记的重组MIH（[(125)I]rMIH）进行了结合试验和化学交联实验。在Y器官的膜部分发现了[(125)I]rMIH的特异性结合（解离常数K(d) = 4.76 x 10(-10) M，最大结合量B(max) = 5.51 x 10(-12) M）。[(125)I]rMIH与Y器官膜部分之间的化学交联显示，MIH受体蛋白的分子大小约为70 kDa。