层粘连蛋白2（α2β1γ1，Lm-211）和层粘连蛋白8（α4β1γ1，Lm-411）由牙髓成纤维细胞合成并分泌，且对三叉神经节感觉神经元的神经突生长有不同程度的促进作用。

Laminins 2 (alpha2beta1gamma1, Lm-211) and 8 (alpha4beta1gamma1, Lm-411) are synthesized and secreted by tooth pulp fibroblasts and differentially promote neurite outgrowth from trigeminal ganglion sensory neurons.

作者信息

Fried Kaj, Sime Wondossen, Lillesaar Christina, Virtanen Ismo, Tryggvasson Karl, Patarroyo Manuel

机构信息

Center for Oral Biology, Department of Odontology, Karolinska Institutet at Huddinge, Stockholm, Sweden.

出版信息

Exp Cell Res. 2005 Jul 15;307(2):329-41. doi: 10.1016/j.yexcr.2005.04.009.

DOI:10.1016/j.yexcr.2005.04.009

PMID:15894315

Abstract

The tooth pulp innervation originates from the trigeminal ganglion (TG) and represents an illustrative example of tissue targeting by sensory nerves. Pulpal fibroblasts strongly promote neurite outgrowth from TG neurons in vitro. In the present study, we have investigated the possible participation of laminins (LNs), potent neuritogenic extracellular matrix components. Immunohistochemistry of human tooth pulp demonstrated expression of LN alpha1, alpha2, alpha4, alpha5, beta1 and gamma1, and laminin-binding integrin alpha3, alpha6, beta1 and beta4 chains in nerves. Though faintly stained for laminins in situ, pulpal fibroblasts reacted, once cultured and permeabilized, with antibodies to LN alpha2, alpha4, beta1 and gamma1 chains by flow cytometry. The cells also expressed the corresponding mRNAs and were able to assemble and secrete LN-2 (alpha2beta1gamma1, Lm-211) and LN-8 (alpha4beta1gamma1, Lm-411). LN-8 displayed a chondroitin sulphate (CS) modification in its alpha4 chain. In functional assays, mouse LN-1 (alpha1beta1gamma1, Lm-111) and recombinant human (rh) LN-8, but not native or rhLN-2, strongly promoted neurite outgrowth from TG neurons, mimicking the effect of cultured pulp fibroblast. Altogether, the results indicate that LN-2 and LN-8 are synthesized by tooth pulp fibroblasts and differentially promote neurite outgrowth from TG neurons. LN-8 may contribute to sensory innervation of teeth and other tissues during development and/or regeneration.

摘要

牙髓神经支配起源于三叉神经节（TG），是感觉神经靶向组织的一个典型例子。牙髓成纤维细胞在体外能强烈促进TG神经元的神经突生长。在本研究中，我们调查了层粘连蛋白（LNs）这一强大的促神经突生长的细胞外基质成分可能发挥的作用。人牙髓的免疫组织化学显示，LN α1、α2、α4、α5、β1和γ1以及层粘连蛋白结合整合素α3、α6、β1和β4链在神经中有表达。虽然牙髓成纤维细胞原位对层粘连蛋白染色较淡，但一旦培养并通透处理后，通过流式细胞术可发现其能与抗LN α2、α4、β1和γ1链的抗体发生反应。这些细胞还表达相应的mRNA，并且能够组装和分泌LN - 2（α2β1γ1，Lm - 211）和LN - 8（α4β1γ1，Lm - 411）。LN - 8在其α4链上有硫酸软骨素（CS）修饰。在功能试验中，小鼠LN - 1（α1β1γ1，Lm - 111）和重组人（rh）LN - 8，而非天然或rhLN - 2，能强烈促进TG神经元的神经突生长，模拟了培养的牙髓成纤维细胞的作用。总之，结果表明LN - 2和LN - 8由牙髓成纤维细胞合成，并差异促进TG神经元的神经突生长。LN - 8可能在发育和/或再生过程中对牙齿及其他组织的感觉神经支配起作用。