鲤（Cyprinus carpio L.）非特异性细胞毒性细胞受体（NCCRP）-1的克隆与分析
Cloning and analysis of non-specific cytotoxic cell receptor (NCCRP)-1 from common carp Cyprinus carpio L.
作者信息
Sakata Hirohiko, Savan Ram, Sogabe Ryo, Kono Tomoya, Taniguchi Keisuke, Gunimaladevi Irengbam, Tassakka Asmi Citra Malina A R, Sakai Masahiro
机构信息
Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen kibanadai-nishi, Miyazaki 889-2192, Japan.
出版信息
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Mar-Apr;140(3-4):287-94. doi: 10.1016/j.cca.2005.02.009.
Nonspecific cytotoxic cell receptor protein (NCCRP-1) provides an important function in target cell recognition and activation of cytotoxicity. NCCRP-1 has been cloned from common carp Cyprinus carpio L. from fish barbel by EST analysis. The isolated gene is composed of 945 bp with a 79 bp 5' UTR, 714 bp open reading frame and 152 bp 3' UTR. The predicted NCCRP-1 gene is composed of 237 amino acid residues and its predicted signal peptide is 19 amino acid residues in length. This gene has conservation of all the related domains characteristic to the NCCRP-1 gene in fish. Phylogenetic and genomic analyses showed that carp NCCRP-1 was similar to other fish orthologues. The expression of NCCRP-1 gene was constitutive in both lymphoid and non-lymphoid tissues. Furthermore, by semi-quantitative RT-PCR studies, we showed that NCCRP-1 gene expression is increased in anterior kidney challenged with Aeromonas hydrophila.
非特异性细胞毒性细胞受体蛋白（NCCRP-1）在靶细胞识别和细胞毒性激活中发挥重要作用。通过EST分析，已从鲤鱼的鱼须中克隆出NCCRP-1基因。分离出的基因由945个碱基对组成，其中5'UTR为79个碱基对，开放阅读框为714个碱基对，3'UTR为152个碱基对。预测的NCCRP-1基因由237个氨基酸残基组成，其预测的信号肽长度为19个氨基酸残基。该基因在鱼类中具有NCCRP-1基因所有相关结构域的保守性。系统发育和基因组分析表明，鲤鱼NCCRP-1与其他鱼类直系同源物相似。NCCRP-1基因在淋巴组织和非淋巴组织中均呈组成型表达。此外，通过半定量RT-PCR研究，我们发现用嗜水气单胞菌攻击前肾后，NCCRP-1基因表达增加。
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