孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）的发情周期阶段非依赖性处理可诱导成年Wistar-Imamichi大鼠超数排卵。

Estrous cycle stage-independent treatment of PMSG and hCG can induce superovulation in adult Wistar-Imamichi rats.

作者信息

Kon Hiroe, Tohei Atsushi, Hokao Ryoji, Shinoda Motoo

机构信息

Laboratory Animal Research Center, Dokkyo University School of Medicine, Tochigi, Japan.

出版信息

Exp Anim. 2005 Apr;54(2):185-7. doi: 10.1538/expanim.54.185.

DOI:10.1538/expanim.54.185

PMID:15897629

Abstract

The estrous cycle influence on the number of ovulated eggs after injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) was investigated in 12, 18, and 24 weeks old adult female Wistar-Imamichi (WI) rats. PMSG (150 IU/kg) was injected at metestrus, diestrus, proestrus, or estrus, followed by hCG (75 IU/kg) 55 h later. Ovulation was induced at all ages and stages of the estrous cycle. The number of ovulated eggs was not affected by stage for similarly aged rats, however, the number of ovulated eggs obtained after treatment decreased with age. These results demonstrate that the PMSG/hCG treatment can induce ovulation at any stage of estrous cycle in WI rats and efficient superovulation at 12 weeks of age.

摘要

研究了12周龄、18周龄和24周龄成年雌性Wistar-Imamichi（WI）大鼠发情周期对注射孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）后排卵卵数的影响。在间情期、动情后期、动情前期或发情期注射PMSG（150 IU/kg），55小时后注射hCG（75 IU/kg）。在发情周期的所有年龄和阶段均诱导排卵。对于年龄相似的大鼠，排卵卵数不受阶段影响，然而，处理后获得的排卵卵数随年龄减少。这些结果表明，PMSG/hCG处理可在WI大鼠发情周期的任何阶段诱导排卵，并在12周龄时实现高效超排卵。