Wu Shang-jie, Zhao Shui-ping
Department of Respiratory disease, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Apr;30(2):207-10.
To investigate the level of mRNA expression of C-reactive proteins (CRP) induced by LPS in human alveolar type epithelial cell line, A549.
Sputum and plasma specimens were obtained from patients with chronic obstructive pulmonary disease (COPD) diagnosed according to the national criteria. CRP was measured by enzyme-linked immunosorbent assays (ELISA). A549 cells were incubated with lipopolysaccharide (LPS) at different concentrations and for different time. CRP mRNA was extracted from cells and the expression was analyzed by RT-PCR. The CRP in the supernatant was measured by ELISA.
The concentration of CRP in the sputum of patients with COPD was significantly higher than that in the plasma (P < 0.05). LPS at different concentrations and for different time induced the expression and secretion of CRP in A549 with a time-dependent increase and the expression of CRP were significantly higher than those of the control (P < 0.05 - 0.01).
Self-secretion of CRP in the respiratory tract may exist. A549 induced by LPS may express CRP mRNA.
研究脂多糖（LPS）诱导人肺泡Ⅱ型上皮细胞系A549中C反应蛋白（CRP）的mRNA表达水平。
从根据国家标准诊断为慢性阻塞性肺疾病（COPD）的患者中获取痰液和血浆标本。采用酶联免疫吸附测定法（ELISA）检测CRP。将A549细胞与不同浓度的脂多糖（LPS）孵育不同时间。从细胞中提取CRP mRNA，通过逆转录聚合酶链反应（RT-PCR）分析其表达。采用ELISA法检测上清液中的CRP。
COPD患者痰液中CRP浓度显著高于血浆（P<0.05）。不同浓度和不同时间的LPS诱导A549中CRP的表达和分泌，呈时间依赖性增加，且CRP的表达显著高于对照组（P<0.05-0.01）。
呼吸道中可能存在CRP的自身分泌。LPS诱导的A549可能表达CRP mRNA。
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